The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Eucommiae Cortex (杜冲) on the Bone Histomorphometry and Metabolism in Ovariectomized Rats

두충(杜冲)이 난소적출(卵巢摘出) 흰쥐의 골조직(骨組織) 형태(形態)와 골대사(骨代謝)에 미치는 영향(影響)

  • Yang, Sung-Woo (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Cho, Jung-Hoon (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Kyung-Sub (Dept. of Gynecology, College of Oriental Medicine, Kyung Hee Univ.)
  • 양성우 (경희대학교 한의과대학 부인과학교실) ;
  • 조정훈 (경희대학교 한의과대학 부인과학교실) ;
  • 장준복 (경희대학교 한의과대학 부인과학교실) ;
  • 이경섭 (경희대학교 한의과대학 부인과학교실)
  • Received : 2005.01.20
  • Accepted : 2005.02.03
  • Published : 2005.02.28
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Abstract

Objective : This study was undertaken to investigate the effects of Eucommiae Cortex on the osteoporosis in ovariectomized rats. Materials and Methods : We used Sprague-Dawley female rats in 8-week-old. They were divided into three groups. Sample group was ovariectomized and administered with 10 mg/100 g/day Eucommiae Cortex extract solution for 10 weeks. Control group was ovariectomized and sham group was conducted by sham's operation. And control group and sham group were administered with normal saline as the same way. We measured rats's body and uterus weight and also measured the serum levels of Ca, phosphorus and ALP. We stained the specimens of rat's tibial bones with Goldner's modified Masson's Trichrome and then examined bone histomorphometry with Bioquant computer program of image analysis system. We measured the thickness of osteoid and callus as static parameters and measured bone volume and mineral apposition rate as dynamic parameters. We observed the expressions of RANKL and OPG mRNA of the tibial bone by RT-PCR. Results : The body weight was significantly (p<0.05) increased in control and sample groups compared with sham group, respectively. The uterus weight was significantly (p<0.05) decreased in control and sample group compared with sham group. In the change of Ca, phosphorus and ALP there were no significant changes among three groups. There were no significant changes of trabecula cortical and osteoid bones' thickness and volume. But trabecula mineral apposition rate (MAR) was significantly (p<0.05) increased in sham and sample group compared with control group. In the expression of RANKL mRNA, sample group was decreased compared with control group, and in that of OPG mRNA, sample group was increased compared with control group. Conclusion : This study shows that Eucommiae Cortex has the beneficial effects on bone histomorphometry and metabolism in the ovariectomized rats. We suggest that Eucommiae Cortex be useful for the treatment of osteoporosis.

Keywords

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