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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Screening of the Antigen Epitopes of Basic Fibroblast Growth Factor by Phage Display

  • Xiang, Junjian (Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University) ;
  • Zhong, Zhenyu (Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University) ;
  • Deng, Ning (Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University) ;
  • Zhong, Zhendong (Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University) ;
  • Yang, Hongyu (Lab of Molecular Immunology and Antibody Engineering in Life Science and Technological College, Jinan University)
  • Published : 2005.05.31
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Abstract

In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.

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References

  1. Baird, A., Schubert, D. and Ling, N. (1988) Receptor and heparin binding domains of basic fibroblast growth factor. Proc. Natl. Acad. Sci. USA 85, 2324-2328 https://doi.org/10.1073/pnas.85.7.2324
  2. Cristopher, A., Nelson, M. and Allen, M. (1986) Human Basic fibroblast growth factor: Nucleitide sequence and genomic organization. EMBO 5, 2523-2528
  3. Kurokawa, M. (1989) Neutralizing antibodies inhibit the binding of basic fibroblast growth factor to its receptor but to heparin. J. Biol. Chem. 246, 7686-7691
  4. Lin, J. N. (1999) The research progress of basic fibroblast growth factor. Chinese J. Bioengineering Progress 19, 25-28
  5. Ray, J., Baird, A. and Gage, F. H. (1997) A 10-amino acid sequence of basic fibroblast growth factor is sufficient for its mitogenic activity on neural progenitor cells. Proc. Natl. Acad. Sci. USA 94, 7047-7052
  6. Reinherz, E. L., Tan, K. and Tang, L. (1999) The crystal structure of a T cell receptor in complex with peptide and MHC class II. Science 286, 1913-1921 https://doi.org/10.1126/science.286.5446.1913

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