Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Enhanced Transduction of Cu,Zn-Superoxide Dismutase with HIV-1 Tat Protein Transduction Domains at Both Termini

  • Eum, Won Sik (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Jang, Sang Ho (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kim, Dae Won (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Choi, Hee Soon (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo Hyun (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kim, So Young (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • An, Jae Jin (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Sun Hwa (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Han, Kyuhyung (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kang, Jung Hoon (Department of Genetic Engineering, Cheongju University) ;
  • Kang, Tae-Cheon (Department of Anatomy, College of Medicine, Hallym University) ;
  • Won, Moo Ho (Department of Anatomy, College of Medicine, Hallym University) ;
  • Cho, Yong Joon (Department of Neurosurgery, College of Medicine, Hallym University) ;
  • Choi, Jin Hi (Research Laboratory of Cell Tech. Korea) ;
  • Kim, Tae Yoon (Department of Dermatology, College of Medicine, Catholic University) ;
  • Park, Jinseu (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo Young (Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University)
  • Received : 2004.10.08
  • Accepted : 2004.12.21
  • Published : 2005.04.30
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Abstract

The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD) is responsible for highly efficient protein transduction across plasma membranes. In a previous study, we showed that Tat-Cu,Zn-superoxide dismutase (Tat-SOD) can be directly transduced into mammalian cells across the lipid membrane barrier. In this study, we fused the human SOD gene with a Tat PTD transduction vector at its N- and/or C-terminus. The fusion proteins (Tat-SOD, SOD-Tat, Tat-SOD-Tat) were purified from Escherichia coli and their ability to enter cells in vitro and in vivo compared by Western blotting and immunohistochemistry. The transduction efficiencies and biological activities of the SOD fusion protein with the Tat PTD at either terminus were equivalent and lower than the fusion protein with the Tat PTD at both termini. The availability of a more efficient SOD fusion protein provides a powerful vehicle for therapy in human diseases related to this anti-oxidant enzyme and to reactive oxygen species.

Keywords

Acknowledgement

Supported by : Korean Ministry of Science and Technology

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