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The Korean Association of Immunobiologists (대한면역학회)
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Alteration of Innate Immune T and B Cells in the NC/Nga Mouse

아토피성 피부질환 동물 모델 NC/Nga 생쥐에서 내재면역 T와 B 세포의 변형

  • Kim, Jung-Eun (Department of Bioscience and Biotechology, Sejong University) ;
  • Kim, Hyo-Jeong (Department of Bioscience and Biotechology, Sejong University) ;
  • Kim, Tae-Yoon (Laboratory of Dermatology and Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea) ;
  • Park, Se-Ho (School of Life Sciences and Biotechnology, Korea University) ;
  • Hong, Seok-Mann (Department of Bioscience and Biotechology, Sejong University)
  • 김정은 (세종대학교 공과대학 생명공학과) ;
  • 김효정 (세종대학교 공과대학 생명공학과) ;
  • 김태윤 (가톨릭대학교 의과대학 피부면역학교실) ;
  • 박세호 (고려대학교 생명공학과) ;
  • 홍석만 (세종대학교 공과대학 생명공학과)
  • Published : 2005.09.30
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Abstract

Background: Millions of people in the world are suffering from atopic dermatitis (AD), which is a chronic inflammatory skin disease triggered by Th2 immune responses. The NC/Nga mouse is the most extensively studied animal model of AD. Like human AD, NC/Nga mice demonstrate increased levels of IgE, a hallmark of Th2 immune responses. Adaptive immunity cannot be generated without help of innate immunity. Especially natural killer T (NKT) cells and marginal zone B (MZB) cells have been known to play important roles in linking innate immunity to adaptive immunity. Methods: Through flow cytometric analysis and ELISA assay, we investigated whether these lymphocytes might be altered in number in NC/Nga mice. Results: Our data demonstrated that the number of NKT cells was reduced in NC/Nga mice and IFN${\gamma}$ production by NKT cells upon ${\alpha}-GalCer$ stimulation decreased to the levels of CD1d KO mice lacking in NKT cells. However, reduction of NKT cells in NC/Nga mice was not due to CD1d expression, which was normal in the thymus. Interestingly, there was a significant increase of $CD1d^{high}B220^+$ cells in the spleen of NC/Nga mice. Further, we confirmed that $CD1d^{high}B220^+$ cells are B cells, not dendritic cells. These $CD1d^{high}B220^+$ B cells show $IgM^{high}CD21^{high}CD23^{low}$, a characteristic phenotype of MZB cells. Conclusion: We provide the evidence that there are decreased activities of NKT cells and increased number of MZB cells in the NC/Nga mice. Our findings may thus explain why NC/Nga mice are susceptible to AD.

Keywords

References

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