Isolation and Genotyping of Enterobacter sakazakii from Powdered Infant Formula Manufactured in Korea

  • Yoo, Mi-Kyung (Department of Food and Nutritional Sciences, Ewha Womans University) ;
  • Kim, Suk-Shin (Department of Food and Nutrition, Catholic University) ;
  • Oh, Sang-Suk (Department of Food and Nutritional Sciences, Ewha Womans University)
  • Published : 2005.12.31

Abstract

Presence of Enterobacter sakazakii, occasional pathogen of powdered infant formula causing rare, but life-threatening diseases such as neonatal meningitis, bacteremia, necrotizing enterocolitis, and necrotizing meningoencephalitis after ingestion was examined in 45 powdered infant formula products manufactured in Korea using chromogenic Druggan-Forsythe-Iversen (DFI) medium, and isolates were identified with API 20E. Ent. sakazakii was isolated from three products. Ent. sakazakii isolates were genotyped by RAPD-PCR using two random primers, and their banding patterns were compared.

Keywords

References

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