Isolation of Hyaluronidase Inhibitory Component from the Roots of Astraglus membranaceus Bunge (Astragali Radix)

  • Lee, Yun-Mi (Department of Biological Engineering, Inha University) ;
  • Choi, Soo-Im (Department of Biological Engineering, Inha University) ;
  • Lee, Jae-Won (Department of Biological Engineering, Inha University) ;
  • Jung, Sun-Mi (Department of Biological Engineering, Inha University) ;
  • Park, Sang-Min (Department of Biological Engineering, Inha University) ;
  • Heo, Tae-Ryeon (Department of Biological Engineering, Inha University)
  • 발행 : 2005.04.30

초록

In order to isolate hyaluronidase (HAase) inhibitor from Astragali radix (AR), dried roots were extracted with ethanol, prior to sequential fractionations with n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. The n-butanol soluble fraction was found to exhibit the most pronounced inhibitory effect (68%) on HAase, and the active components were separated using various chromatographic methods, including column chromatography and preparative HPLC. The active component was isolated from the n-butanol soluble fraction of AR and was structurally identified as calycosin-7-O-${\beta}$-D-glucopyranoside by LC-MS, IR, $^1H$ NMR, and $^{13}C$ NMR analysis. The $IC_{50}$ of calycosin-7-O-${\beta}$-D-glucopyranoside's HAase activity was found to be 3.7 mg/mL.

키워드

참고문헌

  1. Acta Pharm. Sin. v.19 Hypotensive and antiinflammatory effects of Astragalus saponin 1 Zang, Y.D.;Wang, Y.L.;Shen, J.P.;Li, D.X.
  2. Korean J. Pharmacogn. v.32 Inhibitory effect of Astragali Radix on COX-2 activity Kim, E.J.;Oh, O.J.;Lee, S.K.;Yang, K.S.
  3. Korean J. Pharmacogn. v.28 A study on the constituents from the roots of Astragalus memhranaceus Bunge (${\beta}$) Kim, J.S.;Kim, C.S.
  4. Korean J. Pharmacogn. v.31 A study on the constituents from the roots of Astragalus membranaceus Bunge (${\beta}$) Kim, J.S.;Kim, Y.T.;Kim, C.S.
  5. Trends Glycosci. Glycotech. v.8 The hyaluronidase: A chemical, biological and clinical overview Feost, G.I.;Csoka, T.;Stern, R.
  6. Assay for hyaluronidase activity;Methods in Enzymology, Vol. 235 Hynes, W.L.;Ferretti, J.J.
  7. Biochem. Pharmacol. v.17 The effect of anti-inflammatory drugs on the enzyme activity of a rat liver granular fraction high increases vascular permeability Anderson, A.J.
  8. Cancer Res. v.39 Ascorbic acid and cancer: a review Cameron, E.;Pauling, L.;Leibovitz, B.
  9. Arth. Rheum. v.34 Elevated plasma levels of hyaluronic acid in patients with osteoarthritis and rheumatoid arthritis Golberg, R.L.;Huff, J.P.;Lenz, M.E.;Glickman, P.;Katz, R.;Thonar, E.J.M.A.
  10. Korean J. Biotechnol. Bioeng. v.18 Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of tmditional herbal ?medicine extracts Choi, S.I.;Lee, Y.M.;Heo, T.R.
  11. Planta Med. v.59 Inhibitory effects of hydrolysable tannins on $Ca^{+2}$-activated HAase Lee, J.;Lee, S.H.;Min, K.R.;Lee, K.S.;Ro, J.S.;Ryu, J.C.;Kim, Y.
  12. Anal. Biochem. v.109 A convenient plate assay for the quantitation of hyaluronidase in Hymenoptera venoms Richman, P.G.;Baer, H.
  13. J. Chromatogr. v.A 992 Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography Ma, X.;Tu, P.;Chen, Y.;Zhang, T.;Wei, T.;Ito, Y.
  14. J. Chromatogr. v.A 876 Lipid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus Lin, L.Z.;He, X.G.;Lindenmaier, M.;Nolan, G.;Yang, J.;Cleary, M.;Qiu, S.X.;Cordell, G.A.
  15. Korean J. Pharmacogn. v.29 Hyaluronidase inhibitor from Uncariae Ramulus et Uncus Jeong, S.J.;Ko, Y.S.;Ahn, N.H.;Kim, Y.C.
  16. Osteoarthritis Cartilage v.10 Effects of SKI 306X, a new herbal agent, on proteoglycan degradation in cartilage explant culture and collagenase-induced rabbit osteoarthritis model Choi, J.H.;Choi, J.H.;Kim, D.Y.;Yoon, J.H.;Youn, H.Y.;Yi, J.B.;Rhee, H.I.;Ryu, K.H.;Jung, K.;Han, C.K.;Kwak, W.J.;Cho, Y.B.
  17. Chem. Pharm. Bull. v.40 Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions Kakegawa, H.;Matsumoto, H.;Satoh, T.
  18. Planta Med. v.54 Inhibitory effects of hydrangenol derivatives on the activities Kakegawa, H.;Matsumoto, H.;Satoh, T.
  19. Food Sci. Biotechnol. v.12 Mineral Contents and anti-inflammatory effect of ostrich extract combined with Korean herb medicine Kim, E.J.;Yuh, C.S.;Lee, P.J.;Choi, M.K.;Kim, S.Y.