Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Agaricus blazei Murill

  • Chung, Ha-Yull (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University) ;
  • Cho, Young-Jun (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University) ;
  • Kim, Tae-Wook (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University)
  • Published : 2005.04.30
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Abstract

A fraction (CMEx-AH-${\beta}$) of water-soluble polysaccharides, showing selective antitumor activities, was isolated from the mycelia of solid cultured Agaricus blazei Murill by hot-water extraction, ethanol precipitation, and series of chromatography. Chemical characteristics of CMEx-AH-${\beta}$, were as follows: carbohydrate content, 48%; monosaccharide composition, Man:Glu:Gal (2:93:5); molecular weight, $2{\times}10^5$; uronic acid content, 6.2%. Fundamental structure of CMEx-AH-${\beta}$, was deduced as ${\beta}-(1\;{\rightarrow}\;6)$-D-glucan with ${\beta}-(1\;{\rightarrow}\;3)$-D-glucosidic side chains based on results of methylation and $^{13}C$ NMR spectroscopic analyses.

Keywords

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