Biomedical Science Letters (대한의생명과학회지)
- Volume 11 Issue 4
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- Pages.465-471
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- 2005
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
gyrA Mutations Found Among Ofloxacin-resistant Mycobacterium tuberculosis is Isolated from Korea
- Kim Junho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
- Kim Yeun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
- Bae Kiho (Department of Life Science, College of Liberal Arts and Sciences, Yonsei University) ;
- Song Taek-Sun (Department of Microbiology, Yonsei University, College of Medicine) ;
- Cho Sang-Nae (Department of Microbiology, Yonsei University, College of Medicine) ;
- Lee Hyeyoung (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
- Published : 2005.12.01
Abstract
Ofloxacin has antimycobacterial activity that possibly contributes a pivotal role in the second-line drug regimens that are used for the treatment of multidrug-resistant tuberculosis. However, in some communities, the resistance rate of Mycobacterium tuberculosis to this agent is surging. Therefore, a rapid and accurate method that can be used to determine the resistance of M tuberculosis to the ofloxacin can be very useful for effective treatment of the patients. As an effort to develop such a method, this study was set up to reveal general types of mutations that are related to ofloxacin resistance of M tuberculosis. From previous studies, it has been well known that ofloxacin resistance is associated with mutations in a gene encoding the gyrase A subunit protein. In this study, we obtained 43 ofloxacin-resistant and 50 ofloxacin-susceptible M tuberculosis clinical isolates from Masan National TB Hospital, and sequences of DNA fragment of 320 bp, region of gyrA corresponding to the ofloxacin resistance-determining region were analyzed. In brief, the results showed that a total of seven mutation types were found at gyrA. Theses mutations were all clustered within nucleotides 2574 to 2586 of the gyrA gene (codons 88 to 94). Codon 94 was the most frequently substituted site. Twenty-four of the 43 isolates had mutations at this position resulting in a total of five different types of amino acid changes