Characterization of Bacillus licheniformis as a Probiotic

Bacillus licheniformis의 생균제로서의 특성

  • Kim Jin-Wook (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jun Kyoung-Dong (Research and Development Center, Binex Co. Ltd) ;
  • Kang Jae-Seon (Department of Pharmaceutical Engineering, Inje University) ;
  • Jang Jung-Su (Research and Development Center, Binex Co. Ltd) ;
  • Ha Bae-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 김진욱 (신라대학교 공과대학 생명공학과) ;
  • 전경동 (㈜ 바이넥스 중앙연구소) ;
  • 강재선 (인제대학교 제약공학과) ;
  • 장정수 (㈜ 바이넥스 중앙연구소) ;
  • 하배진 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Published : 2005.10.01

Abstract

Bacillus licheniformis was an useful material for improvement of fecal microflora. The stability of Bacillus licheniformis in low pH (pH 2, 3, 4, 6, 7), artificial bile acid, high concentration of salt (0, 5, 10, 20, $30\%$), and ethanol (0, 4, 8, 16, $32\%$) was investigated in this study. The viability of Bacillus licheniformis was stable for pH 3, 4, 6, 7 Final viability of Bacillus licheniformis was confirmed below $25\%$, in the bile acid. In high concentration of salt, Bacillus licheniformis showed optimal viability in $10\%$ of salt. The viability of Bacillus licheniformis was over $95\%$ in condition of ethanol concentration $4\%,\;8\%,16\%,\;32\%$ contrast with uncontained ethanol at $37^{\circ}C$ for 4h. It was verified that Bacillus licheniformis was stable for pH 4, 6, 7, high concentration of salt and ethanol but was unstable in pH 2 and bile acid.

Bacillus licheniformis는 장내 균총을 개선하는데 유용한 물질이다. 본 연구의 목포는 B. licheniformis의 낮은 pH (pH 2, 3, 4, 6, 7), 인공 담즙산, 높은 농도의 염 (0. 5, 10, 20, $30\%$) 그리고 에탄올 (0, 4, 3, 16, $32\%$)에 대한 안정성을 확인하려고 한다. B. licheniformis는 pH 3, 4, 6, 7에서 안정적인 생존율을 보였다. B. llicheniformis의 담즙 내에서의 최종 생존율은 $25\%$ 이하로 확인되었다. 높은 농도의 염에서는 B. licheniformis는 $10\%$의 염에서 최적 생존율을 보였다. licheniformis의 에탄올이 포함되지 않은 것에 비해 에탄올이 4, 8, 16, $32\%$ 포함된 것에서 $95\%$ 이상의 생존율을 보였다. 그러므로 B. licheniformis가 pH 3, 4, 6, 7, 높은 농도의 염, 에탄올에서 안정하나 pH 2와 담즙산에서 불안정하다는 것을 확인할 수 있었다.

Keywords

References

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