KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of Crude $\alpha-fucosidase$ from Bacteroides fragilis Roid8

Bacteroides fragilis Roid8의 $\alpha-fucosidase$ 조효소 특성

  • Heo Soon Young (Department of Food and Nutrition, Seoul National University) ;
  • Park Myung Soo (Research Center, BIFIDO Co. Ltd) ;
  • Kwon Bin (Department of Food and Nutrition, Seoul National University, Research Center, BIFIDO Co. Ltd) ;
  • Ji Geun Eog (Research Institute of Human Ecology, Seoul National University)
  • 허순영 (서울대학교 식품영양학과) ;
  • 박명수 (㈜비피도 기술연구소) ;
  • 권빈 (서울대학교 식품영양학과, ㈜비피도 기술연구소) ;
  • 지근억 (서울대학교 생활과학연구소)
  • Published : 2005.10.01
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Abstract

Bacteroides is the most predominant bacteria in the human large intestinal tract. Bacteroides fragilis RoidB isolated from a Korean faecal sample showed high $\alpha-fucosidase$ activity compared to other bacterial species. The optimized meidum for the production of $\alpha-fucosidase$ from 8. fragilis contained BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K1mg, and starch 5g per 1 L. The crude $\alpha-fucosidase$ obtained through DEAE-sepharose and CM- cellulose chromatography showed optimum temperature and pH at $40^{\circ}C$ and 7.0, respectively. Among several metals, $Co^{++}\;and\;Zn^{++}$ showed strong inhibition on enzyme activity.

Bacteroides fragiles Roid8은 인간의 장내에서 가장 우세한 균종에 속하는 것으로서 효소활성을 측정해본 결과 다른 균들에 비해 $\alpha-fucosidase$의 활성이 높은 것으로 나타났다. 본 연구실에서 조사된 $\alpha-fucosidase$의 생산 최적배지 조성은 1L 배지당 BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K 1mg, starch 5g을 함유하였다. 또한 DEAE-sepharose와 CM-cellulose 크로마토그래피를 거쳐 조효소액을 분리하여 조사한 결과 효소의 최적 반응온도는 $40^{\circ}C$, 최적반응 pH는 7.0이었다. 무기 금속이온의 영향은 $Co^{++}\;and\;Zn^{++}$ 존재 하에서 효소역가가 낮아졌다.

Keywords

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