생물환경조절학회지 (Journal of Bio-Environment Control)

  • 제14권4호
  • /
  • Pages.298-301
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  • 2005
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  • 1229-4675(pISSN)
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  • 2765-3641(eISSN)
한국생물환경조절학회 (The Korean Society for Bio-Environment Control)
권호 표지

Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

  • Choi Ki Young (Northern Kyungbuk Resion Bio Industry Innovation Center, Dong Yang University) ;
  • Son Sung Ho (Vitrosys, Co. Ltd) ;
  • Lee Joo Hyun (Department of Environmental Horticulture, The University of Seoul) ;
  • Lee Yong-Beom (Department of Environmental Horticulture, The University of Seoul) ;
  • Bae Jong Hyang (Division of Plant and Resource Science, Wonkwang university)
  • 발행 : 2005.12.01
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초록

Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

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