KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Plant Regeneration from Sliced Mature Embryo Fragments of Wheat Cultivars

  • Kim Kyung-Hee (Department of Plant Biotechnology, Dongguk University) ;
  • Kang Moon-Seok (National Institute of Crop Science, RDA) ;
  • Kwon Young-Up (National Institute of Crop Science, RDA) ;
  • Lee Sang-Kyu (Department of Plant Biotechnology, Dongguk University) ;
  • Moon Jung-Hun (Department of Plant Biotechnology, Dongguk University) ;
  • Han Sinae (Department of Plant Biotechnology, Dongguk University) ;
  • Oh Poo-Reum (Department of Plant Biotechnology, Dongguk University) ;
  • Lee Byung-Moo (Department of Plant Biotechnology, Dongguk University)
  • Published : 2005.12.01
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Abstract

Mature embryos were aseptically excised with a scalpel and sliced in fragments measuring 0.5 mm in diameter (sliced mature embryo fragment; 4 ${\~}$ 5 fragments/one embryo). Sliced mature embryo fragments of six wheat cultivars were cultured to develop an efficient method of callus induction and plant regeneration. Callus derived from sliced mature embryo fragments showed a good capacity to embryogenesis and regeneration. Furthermore sliced mature embryo fragments decreased contamination from fungi and bacteria. The high efficiency of callus induction were obtained Keumkangmil and Bob­white. For plant regeneration, selected embryogenic calli were transferred to two types regeneration media. An average number of green spots per callus was 4 to 5 in regeneration media after about one week. Percentage of plant regeneration showed high in regeneration medium containing 0.1 mg/l 2,4-D and 5 mg/l zeatin. Especially, Keumkangmil ($27.5\%$) and Bobwhite ($33.3\%$) showed high regeneration efficiency. This regeneration system from sliced mature embryo fragments may provide an effective and convenient explant for plant transformation studies.

Keywords

References

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