Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
권호 표지

Tobacco Transformation Using Expansin Genes

Expansin 유전자를 이용한 담배의 형질전환

  • Choi Dongsu (Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University) ;
  • Kim Ho-Bang (School of Biology, Seoul National University) ;
  • Kim Jeong-Hoe (Department of Biology, Kyungpook National University) ;
  • Shin Ju-Sik (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Seok Yeong-Seon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung Chan-Moon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 최동수 ;
  • 김호방 (서울대학교 자연과학대학 생명과학부) ;
  • 김정회 (경북대학교 자연과학대학 생물학과) ;
  • 신주식 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 석영선 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 정찬문 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 이이 (충북대학교 농업생명환경대학 특용식물학과)
  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.

Keywords

References

  1. Belfield, E. J., Ruperti, B., Roberts, J. A. and McQueen-Mason, S. (2005) Changes in expansin activity and gene expression during ethylene-promoted leaflet abscission in Sambucus nigra. J. Exp. Bot. 56: 817-823 https://doi.org/10.1093/jxb/eri076
  2. Brummell, D. A., Harpster, M. H., Civello, P. M., Palys, J. M., Bennett, A. B. and Dunsmuir, P. (1999) Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening. Plant Cell 11: 2203-2216 https://doi.org/10.1105/tpc.11.11.2203
  3. Cho, H-T. and Cosgrove, D. J. (2000) Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA. 97: 9783-9788
  4. Cho, H-T. and Cosgrove, D. J. (2002) Regulation of root hair initiation and expansin gene expression in Arabidopsis. Plant Cell 14: 3237-3253 https://doi.org/10.1105/tpc.006437
  5. Choi, D., Lee, Y., Cho, H-T. and Kende, H. (2003) Regulation of expansin gene expression affects growth and development in transgenic rice plants. Plant Cell 15: 1386-1398 https://doi.org/10.1105/tpc.011965
  6. Cosgrove, D. J. (1999) Enzymes and other agents that enhance cell wall extensibility. Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 50:391-417 https://doi.org/10.1146/annurev.arplant.50.1.391
  7. Cosgrove, D. J. (2000a) Loosening of plant cell walls by expansins. Nature 407:321-326 https://doi.org/10.1038/35030000
  8. Cosgrove, D. J. (2000b) New genes and new biological roles for expansins, Curr. Opin. Plant. Biol. 3:73-78 https://doi.org/10.1016/S1369-5266(99)00039-4
  9. Cosgrove, D. J., Bedinger, P. and Durachko, D. M. (1997) Group I allergens of grass pollen as cell wall-Ioosening agents. Proc. Natl. Acad. Sci. USA. 94: 6559-6564
  10. Gookin, T. E., Hunter, D. A. and Reid, M. S. (2003) Temporal analysis of alpha and betaexpansin expression during floral opening and senescence. Plant Sci. 164: 769-781 https://doi.org/10.1016/S0168-9452(03)00063-3
  11. Hiwasa, K., Rose, J. K. C., Nakano, R., Inaba, A. and Kubo, Y. (2003) Differential expression of seven $\alpha$-expansin genes during growth and ripening of pear fruit. Physiol. Plant. 117: 564-572 https://doi.org/10.1034/j.1399-3054.2003.00064.x
  12. Huelsenbeck, J. P. and Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754-755 https://doi.org/10.1093/bioinformatics/17.8.754
  13. Kende, H., Bradford, K. J., Brummell, D. A., Cho, H-T., Cosgrove, D. J., Fleming, A. J., Gehring, C., Lee, Y., McQueen-Mason, S. J., Rose, J. K. C. and Voesenek, L. A. C. J. (2004) Nomenclature for members of the expansin superfamily of genes and proteins. Plant Mol. Biol. 55: 311-314 https://doi.org/10.1007/s11103-004-0158-6
  14. Kitagawa, M., Ito, H., Shiina, N., Nakamura, N., Inakuma, T., Kasumi, T., Ishiguro, Y., Yabe, K. and Ito, Y. (2005) Characterization of tomato fruit ripening and analysis of gene expression in F1 hybrids of the ripening inhibitor (rin) mutant. Physiol. Plant. 123: 331-338 https://doi.org/10.1111/j.1399-3054.2005.00460.x
  15. Lee, Y. and Choi, D. (2005) Biochemical Properties and Localization of the $\beta$-Expansin OsEXPB3 in Rice (Oryza sativa L.). Mol. Cells 20: 119-126
  16. Lee, Y., Choi, D. and Kende, H. (2001) Expansins: ever-expanding numbers and functions. Curr. Opin. Plant. Biol. 4: 527-32 https://doi.org/10.1016/S1369-5266(00)00211-9
  17. Lee, Y. and Kende, H. (2001) Expression of $\beta$-expansins is correlated with internodal elongation in deepwater rice. Plant Physiol. 127: 645-654 https://doi.org/10.1104/pp.010345
  18. Lee, Y. and Kende, H. (2002) Expression of $\alpha$-expansin and expansin-like genes in deepwater rice. Plant Physiol. 130: 1396-1405 https://doi.org/10.1104/pp.008888
  19. Li, Y., Darley, C. P., Ongaro, V., Fleming, A., Schipper, O., Baldauf, S. L. and McQueenMason, S. J. (2002) Plant expansins are a complex muitigene family with an ancient evolutionary origin. Plant Physiol. 128: 854-864 https://doi.org/10.1104/pp.010658
  20. McQueen-Mason, S., Durachko, D. M. and Cosgrove, D. J. (1992) Two endogenous proteins that induce cell waIl expansion in plants. Plant Cell 4: 1425-1433 https://doi.org/10.1105/tpc.4.11.1425
  21. Pien, S., Wyrzykowska, J., McQueen-Mason, S. J., Smart, C. and Fleming, A. (2001) Local expression of expansin induces the entire process of leaf development and modifies leaf shape. Proc. Natl. Acad. Sci. USA. 98: 11812-11817
  22. Sampedro, J., Lee, Y., Carey, R. E., dePamphilis, C. and Cosgrove, D. J. (2005) Use of genomic history to improve phylogeny and understanding of births and deaths in a gene family. Plant J. 44: 409-419 https://doi.org/10.1111/j.1365-313X.2005.02540.x
  23. Sasaki, T. and Burr, B. (2000) International rice genome sequencing project: the effort to completely sequence the rice genome. Curr. Opin. Plant. Biol. 3: 138-141 https://doi.org/10.1016/S1369-5266(99)00047-3
  24. Schmitt, F., Oakeley, E. J. and Jost, J. P. (1997) Antibiotics Induce Genome-wide Hypermethylation in Cultured Nicotiana tobacum Plants. J. Biol. Chem. 272: 1534-1540 https://doi.org/10.1074/jbc.272.3.1534
  25. Shin, J. H., Jeong, D. H., Park, M. C. and An, G. (2005) Characterization of $\alpha$-expansin gene family in rice. Mol. Cells 20: 210-218
  26. The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815 https://doi.org/10.1038/35048692