Tobacco Transformation Using Expansin Genes

Expansin 유전자를 이용한 담배의 형질전환

  • Choi Dongsu (Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University) ;
  • Kim Ho-Bang (School of Biology, Seoul National University) ;
  • Kim Jeong-Hoe (Department of Biology, Kyungpook National University) ;
  • Shin Ju-Sik (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Seok Yeong-Seon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung Chan-Moon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 최동수 ;
  • 김호방 (서울대학교 자연과학대학 생명과학부) ;
  • 김정회 (경북대학교 자연과학대학 생물학과) ;
  • 신주식 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 석영선 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 정찬문 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 이이 (충북대학교 농업생명환경대학 특용식물학과)
  • Published : 2005.12.01

Abstract

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.

Keywords

References

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