Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Isolation and Identification of Stenotrophomonas maltophilia BW-13 Active Against Rhizoctonia solani Causing Crisphead Lettuce Bottom Rot

Rhizoctonia solani에 의한 결구상추 밑둥썩음병 방제균주 Stenotrophomonas maltophilia BW-13의 분리 및 동정

  • Kim Han-Woo (Center for Biotechnology, Dong-A University) ;
  • Park Jong-Young (Division of Applied Biology, Dong-A University) ;
  • Kim Hyun-Ju (Division of Applied Biology, Dong-A University) ;
  • Lee Kwang-Youll (Division of Applied Biology, Dong-A University) ;
  • Lee Jin-Woo (Division of Applied Biology, Dong-A University) ;
  • Choi Woobong (Dept. Biotechnology and Bioengineering, Dong-Eui University) ;
  • Lee Seon-Woo (Division of Applied Biology, Dong-A University) ;
  • Moon Byung-Ju (Division of Applied Biology, Dong-A University)
  • 김한우 (동아대학교 농업생명연구소) ;
  • 박종영 (동아대학교 응용생물공학부) ;
  • 김현주 (동아대학교 응용생물공학부) ;
  • 이광렬 (동아대학교 응용생물공학부) ;
  • 이진우 (동아대학교 응용생물공학부) ;
  • 최우봉 (동의대학교 생명공학과) ;
  • 이선우 (동아대학교 응용생물공학부) ;
  • 문병주 (동아대학교 응용생물공학부)
  • Published : 2005.12.01
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Abstract

In a course of searching for biofungicide to control crisphead lettuce bottom rot caused by Rhizoctonia solani, we have isolated an antagonistic bacterium from lettuce rhisophere soil. A total of 702 bacterial isolates were isolated and tested for in vitro growth inhibition of R. solani. Seven strains appeared to have strong antagonistic effect against R. solani in in vitro growth inhibition assay. In the pot experiments, a strain BW-13 showed the most potent disease control effect on the both lettuce seedlings and adults plants. Therefore, the BW-13 was selected as a biocotrol candidate against crisphead lettuce bottom rot. Based on its morphology, physiological characteristics, and 165 rRNA gene analysis, the BW-13 was finally identified as Stenotrophomonas maltophilia. This study indicated that S. maltophilia BW-13 could be used as a biocontrol agent to control crisphead lettuce bottom rot.

결구상추 밑둥썩음병의 병원균 Rhizoctonia solani에 대한 길항세균을 분리하기 위하여 결구상추 재배 포장에서 결구상추의 밑둥부위, 뿌리조직 그리고 근권토양에서 세균 702균주를 분리하였다. 분리균주 중에서 대치배양에 의해 길항능을 나타낸 7균주를 선발하였으며, 그 중 포트검정 실험에서 유묘와 성체식물에서 발병억제효과가 우수한 BW-13균주를 길항균으로 최종선발하였다. 최종 선발된 BW-13균주의 동정을 위하여 생리$\cdot$생화학적 특성, API test 및 16S rDNA sequence를 분석한 결과 Stenotrophomonas maltophilia로 동정되었으며, 본 균주를 S. maltophilia BW-13으로 명명하였다. 본 연구의 결과는 S. maltophilia BW-13이 결구상추 밑둥썩음병의 생물적 방제원으로 이용될 수 있음을 보여준다.

Keywords

References

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