Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Characterization of Potato Blackleg Strains from Jeju Island

제주지역에서 분리한 감자 줄기검은병균의 유전적 특성

  • Seo Sang-Tae (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Lee Seungdon (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee Jung-Sup (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Han Kyoung-Suk (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Jang Han-Ik (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Lim Chun-Keun (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University)
  • 서상태 (원예 연구소 원예환경과) ;
  • 이승돈 (농업과학기술원 식물병리과) ;
  • 이중섭 (원예 연구소 원예환경과) ;
  • 한경숙 (원예 연구소 원예환경과) ;
  • 장한익 (원예 연구소 원예환경과) ;
  • 임춘근 (강원대학교 농생물학과)
  • Published : 2005.12.01
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Abstract

A collection of 12 Erwinia carotovora strains from blackleg diseased potato in Jeju island was characterized genetic diversity by 5. cayotovora subsp. atposeptica (Eca)-specific PCR, PCR-RFLP of the two genes (16S rRNA and pel) and repetitive sequence PCR (ERIC-PCR). The results were compared with those of the other E. carotovora representative strains. None of the blackleg strains produced PCR amplicons with Eca-specific primers in contrast to the single 690 bp amplicon obtained with Eca strains. In addition, on the basis of pel gene RFLP with Sau3AI, the blackleg strains belonged to the pattern 2 whereas Eca strains belonged to the other one (pattern 3). By analysis of 16S rDNA RELP generated with HinfI, the most strains including the E. carotovera subsp. carotovora (Ecc) representative strains used in this study belonged to the pattern 1 whereas the blackleg strains belonged to the pattern 2 except for one strain. Moreover, ERIC-PCR analysis showed that the blackleg strains were closely related to each other and had an unique DNA band. Based on these molecular approaches, we have confirmed that the blackleg disease of potato is caused by a different E. carotovora from Eca and Ecc in Jeju island.

제주지역의 감자 줄기검은병징으로부터 분리한 12균주의 유전적 특성을 분석하기 위해 Eca-specific PCR, PCR-RFLP, ERIC-PCR을 실시하여 그 결과를 E. carotovora대조균들과 비교하였다. Eca-specific PCR 결과 Eca 대조균들은 특이적 밴드를 형성한 반면, 줄기검은병균은 특이적 밴드를 형성하지 않았다. 또한, pel유전자의 RFLP분석 결과 줄기검은병균은 pattern 2를 나타내었으나, Eca 균주는 pattern 3을 나타내어 Eca와는 다른 특성을 보여주었다. 16S rDNA의 RFLP분석결과 이번 실험에 이용된 대부분의 균주가 pattern 1을 나타냈지만, 12개의 줄기검은병균 중 11개의 균이 pattern 2을 나타내어 Ecc와도 다른 특성을 보여주었다. 제주지역의 무름병징과 줄기검은병징을 나타내는 균주들의 유전적 관계를 분석하기 위해 ERIC-PCR을 실시한 결과 줄기검은병균들은 특이적 밴드를 형성하였으며, 서로 높은 유연관계를 보여주었다. 따라서 제주지역의 줄기검은병징으로부터 분리한 균주들은 Eca, Ecc균들과는 다른 특성을 가지고 있음을 알 수 있었다.

Keywords

References

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