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서양금혼초(Hypochaeris radicata L.)의 수용추출물이 사료작물에 미치는 allelopathy 효과

The Allelopathic Effects of Aqueous Extracts of Hypochaeris radicata L. on forage Crops

  • 김옥임 (동아대학교 생명자원과학대학) ;
  • 박선일 (동아대학교 생명자원과학대학) ;
  • 정일민 (건국대학교 식량자원학과) ;
  • 하상영 (동아대학교 생명자원과학대학)
  • Kim Og-Yim (Department of Science, College of Natural Resources and Life Sciences, Dong-A University) ;
  • Park Sun-Ill (Department of Science, College of Natural Resources and Life Sciences, Dong-A University) ;
  • Jung Ill-Min (College of Life and Environment Science, Konkuk University) ;
  • Ha Sang-Young (Department of Science, College of Natural Resources and Life Sciences, Dong-A University)
  • 발행 : 2005.12.01

초록

서양금혼초(Hypochaeris radicata Linne)에 함유되어있는 화학물질이 사료작물의 미치는 allelopathy효과를 구명하기 위하여 본 실험을 실시하였다 서양금혼초에 함유되어 있는 allelochemicals 물질을 HPLC에 의해 성분 분석한 결과 계절적, 부위별로 차이가 있었으나 $\rho$-hydroxybenzoic acid, chlorogenic acid, catechin, caffeic acid, syringic acid, salicylic acid, $\rho$-coumaric acid, ferulic acid, naringin, hesperidin, myricetin, krans-cinnamic acid, quercetin, naringenin 등 14 종류의 phenolic compounds 성분을 확인하였고 caffeic acid, ferulic acid, naringenin 3종류는 공통으로 함유하고 있는 것으로 분석되었다. 5월에는 일, 7월에는 꽃과 뿌리에서 많은 양의 phenolic acids가 함유되어있고 특히 뿌리에 sali-cylic acid $2085.6{\mu}g/g$,와 quercetin $1522.0[\mu}g/g$,로 함량이 높았다. 서양금혼초 수용성 추출액으로 처리한 사료작물의 생장을 조사한 결과 품종에 따라 처리구와 대조구의 값이 같은 경우도 있으나 모든 조사 항목에서 대조구 보다 처리 값이 낮아 사료작물의 생장이 억제 된 것이 확인되었고 처리농도가 높을수록 강한 억제효과를 나타내었다. 수량의 변화를 조사한 결과 사료작물 품종에 따라 차이가 있으나 전체적으로 억제효과를 나타내었으며 처리농도가 높을수록 강한 억제효과를 나타내었다. 이러한 결과 서양금혼초는 사료작물에 대한 allelopathy 효과가 있음을 밝혀냈다. 특히 화본과 사료작물의 경우 italian ryegrass, 콩과 사료작물의 경우는 purple alfalfa가 생장억제효과가 높게 나타났다.

This experiment was performed to examine the allelopathy effect of allelochemical substance on the crop plants. According to the experiment of the allelochemical substances in Hypochaeris radicata by HPLC, there are the differences at each part of plants. However, it is ascertained that there are 14 kinds of phenolic compounds ingredients that are $\rho$-hydroxybenzoic acid, chlorogenic acid, catechin, caffeic acid, syringic acid, salicylic acid, $\rho$-coumaric acid, ferulic acid, naringin, hesperidin, myricetin, trans-cinnamic acid, quercetin and naringenin. The chemicals like caffeic acid, ferulic acid, and naringenin are commonly included. The result of the chemical experiment shows that there are the differences at each season and part of plants. The leaves in May and blossoms and roots in July contain lots of phenolic acids. It is very high contents such as salicylic acid 2085.6 ${\mu}g/g$ and quercetin 1522.0 ${\mu}g/g$, especially in roots of plants. The result on the growth of crop plants treated by the aqueous extract of Hypochaeris radicata shows that the value of the control group and the test group are same in some cases. However, because the treat value of test group is towel'than that of control group in all items of the experiment, it is cofirmed that the growth of crop plants was inhibited and that the inhibitory effect was increased as its density of treatment was increased. The result of change in quantity shows that there are the differences at each kind of crop plants, but the inhibitory effect was increased as its concentration of treatment was increase with entire. As results, it is confirmed that H. radicata has the allelopathy effect to the crop plants. Especially the inhibitory effect on growth is high in gramineous crop, italian ryegrass and leguminous crop. purple alfalfa.

키워드

참고문헌

  1. Inderjit. 1996. Plant phenolics in allelophy. Bot. Rev. 62, 182-210
  2. Molish, H. 1937. Der Eimfluss einer Pflanze auf die andere Allelopathie. Fischer Jena 20
  3. Newman, E. I. 1978. Allelopathy: Adaptation on accident. pp. 327-342. In Harbone, J. B .(ed.), Biochemical Aspects of Plant and Animal Coevalution. Acad. press, New York
  4. Kim, Y. O. and H. J. Lee. 1996. Identification and effects of phenolic compounds from some plants. Kor. J. Ecol. 19, 329-340
  5. Ho.-Joon. Lee., Yong-O.k Kim and Nam.-Kee. Chang. 1997. Allelopathic effects on seed germination and fungus growth from the secreting substances of some plants. Kor. J. Ecol. 20(3), 181-189
  6. Rice, E. L. 1984. Alldopathy. 2nd ed. Academic Press, New York and London
  7. Aber, J. D. and J. M. Melillo. 1991. Terrestrial ecosystems, pp. 315-316, Saunder Collage Pub
  8. Newsome, A. E. and I. R. Noble. 1986. Ecological and physiological characteristics of invading species, pp. 1-33, In R. H. Groves and J. J. Burdonc (eds.), Ecology of Biological Invasions, Cambridge Univ. Press
  9. Rejmane, K. M. and D. M. Richardson. 1996. What attributes make some plant species more invasive. Ecology 77, 1655-1661 https://doi.org/10.2307/2265768
  10. Curran, P. L. and F. S. Acuaeidhe. 1986. Weed invasion of milled-over bog. Weed Res. 26, 45-50 https://doi.org/10.1111/j.1365-3180.1986.tb00675.x
  11. Forcella, F. 1985. Final distribution is related to rate of speed in alien weed. Weed Res. 25, 181-191 https://doi.org/10.1111/j.1365-3180.1985.tb00634.x
  12. Ackowiak, B. 1996. Chrologial-ecological model of the spread of Puccindla distans (Poaceae) in Central Europe. Firagm. Flor. Cabot. 41, 551-561
  13. Kil, B. S., E. S. Jeon, Y. S. Kim, C. H. Kim, K. W. Yun, H. G. Yoo, B. S. Kim and H. C. Kim. 1998. Flora and Distribution of Mt. Nam Park, Seoul. Kor. J. Ecol. 21, 603-631
  14. Kim, Y. O., Park. J. Y and H. J. Lee. 2003. Environmental Adaptability of Eupatorium rugosum: Relationship between accumulation of heavy metals and phenolic compounds. Kor. J. Ecol. 26(1), 5-12 https://doi.org/10.5141/JEFB.2003.26.1.005
  15. Pyse K, P. and K. Prach. 1993. Plant invasion and the role of riparian habitats: a composition of four species alien to central Europe. J. Biogeogr. 20, 413-420 https://doi.org/10.2307/2845589
  16. Jin, H. Z., and J. H. Kim. 2001. Allelopathic effects of volatile compounds from Ambrosia artemisiifolia leaves on the selected species. Kor. J. Ecol. 24(1), 61-66
  17. Lee, H. J., Y. O. Kim and E. J. Lee. 2000. Antimicrobial activities of extracts from several native and exotic plants in Korea. Kor. J. Ecol. 23(5), 353-357
  18. Lee, H. J., S. H. Kim, Y. O. Kim and M. Y. Eun. 1990. Allelopathic Effects of Leaf Extract of Pinus rigida Mill, on the seeds germination of Raphanus sativus var. hortensis for acanthiformis Makino. Kor. J. Ecol. 13(2), 75-82
  19. Lee, H. J., Kim, Y. O. Kim, I. T. and Lee, J. H. 2001. Allelopathic effects of extracts of Trifolium repens on the seed germination and seedling growth of Zoysia japonica. Kor. J. Ecol. 24(3), 125-130
  20. Yu, C. Y., I. S. Jeon, I. M. Chung, J. H. Hur and E. H. Kim. 1995. Research Reports: The allelopathic effect of alfalfa residues on crops and weeds. Kar. J. Weeds. Sci. 15(2), 131-140
  21. Kil, B. S. 1993. Effects of naturally occurring chemicals from Pinus koraiensis on callus induction and gerrniculture. Kor. J. Ecol. 16(3), 275-285
  22. Kim, Y. O. 1995. Effect of leaf extract from pinus rigida on morphological changes of root tips. J. Plant. BioI. 38(1), 73-78
  23. Rice, E. L. 1984. Allelopathy. pp. 422, 2nd ed., Academic Press, New York and London
  24. Kim, H. Z. and Kim, J. H. 2001a. The allelopathic effects of aqueous chemicals of Ambrosia artemisiifolia on selected plants. Kor. J. Ecol. 24(1), 64-73
  25. Kil, B. S and K. W. Yun. 1992. Allelopathic effects of water extracts of Artemisia primceps var. orientalis on selected plant species. J. Chem. Ecol. 18, 39-51 https://doi.org/10.1007/BF00997163
  26. Yun, K. W., B. Vir and D. H. Han. 1993. Phytotoxic and antimicrobial activity of volatile constituents of Artemisia priceps var. orientalis. J. Chem. Ecole. 19, 2757-2767 https://doi.org/10.1007/BF00980705
  27. Al-Naib, F. A. and E. L. Rice. 1971. Alleopathic effect of Platanus occidentalis Bull. Torrey Bot. Club 98: 75-82 https://doi.org/10.2307/2483770
  28. Kuo, C. G., M. H. Chou and H. G. Park. 1981. Effect of chinese cabbage residue on mungbean. Plant and Soil. 61: 473-477 https://doi.org/10.1007/BF02182027
  29. Lee, J. H. and Lee, S. R. 1994. Analysis of phenolic substances content in korean plant foods. J. Food Sci. Technol. 26, 310-316
  30. Wieslaw, O, Lee, C. Y, Antoni, W and Price, K. R. 1988. Identification of some phenolic compounds in apples. J. Agric. Food Chem. 36, 430-432 https://doi.org/10.1021/jf00081a007
  31. W. L. Banwart, P. M. Porter, T. C. Granato and J. J. Hassett. 1985. HPLC .separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. Joural of Chemical Ecology. 11(3), 383-395 https://doi.org/10.1007/BF01411424
  32. Lodhi, M. A. K. 1976. Kolo of allelopathy as expressed by dominating tree in a low land forest in controlling the productivity and pattern of herbaceous growth. Am J. Bot. 63, 1-8 https://doi.org/10.2307/2441664
  33. Blum, U. and B, R. Dalton. 1985. Effect of ferulic acid, an allelopathic compound, one leaf expansion of cucumber seedling grown in nutrient culture. J. Chem. Ecol. 11, 279-302 https://doi.org/10.1007/BF01411415
  34. Einhellig, F. A. and J. A. Rasmussen. 1973. Allelopathic effects of Rumex crispus on Amaranthus retroflexus grain sorghum and field corn. Amer. Mid. Nat. 90, 79-86 https://doi.org/10.2307/2424268
  35. William, R. D. and R. E. Hloagland. 1982. The effects of naturally occuring phenolic compounts on seed germination. Weed Sci. 30, 206-212
  36. Baldwin, I. T. and P. Colleham. 1993. Autotoxicity and chemical defense: nicotine accumulation and carbon gain in solanaceous plants. Oecologia 94: 534-541 https://doi.org/10.1007/BF00566969
  37. Kil, B. S. and H. G. Yoo. 1996. Identification and growth inhibition of phytotoxic substances from Artemisia scoparia. Kor. J. Ecol. 19, 295-304
  38. Rajeswara, R, P. N. Kaul, G. R. Mallavarepu and S. Ramesh. 1996. Effects of seasonal climatic changes on biomass yield and terpenoid composition of Rosescented geranium. Biochemical Syst. and Ecool. 24: 627-636 https://doi.org/10.1016/S0305-1978(96)00071-3
  39. Thompson, A. C. 1985. The chemistry of allelopathy, biochemical interactions among plants. Am. CHem. Soc. Symp. Ser. 268
  40. Fischer, N. .H. 1991. Plant terpenoids as allelopathic agents. pp. 377-398, In J. B. Harbome and F. A. TomasBarberan (eds.), Ecological chemistry and biochemis of plant terpenoids. Clarendon press. Oxford
  41. Inderjit, K. M. and M. Dakshini. 1992 Interference potential of Pluched lanceolata (Asteraceae): Growth and physiological responses of asparagus bean, Vigna unguiculata var. sesquipendalis. Am J. Botany. 79(9), 979-981
  42. Baskin, J. M. and F. T. Wilf. 1967 Psoraen, an inhibition in the seeds of Psoralea subaculis (Leguminosae). Phytochemistry. 6, 1209-1213 https://doi.org/10.1016/S0031-9422(00)86083-5
  43. Rice, E. L. and S. K. Pancholy. 1974. Inhibition of nitrification by climax ecosystems. III. Inhibitors other than tannins. Am. J. Bot. 61, 1095-1103 https://doi.org/10.2307/2441927
  44. Yun, K. W. and M. A. Maun. 1997 Allelopathic potential of Artemisia campestris ssp. caudata on lake Huron sand dunes. Can. J. Bot. 75, 1903-1912 https://doi.org/10.1139/b97-902
  45. Kaori, Y., N. Goto, S. Kosemura and K. Hasegawa. 1997. Growth promoting allelopathic substance exuded from geminating Arabidopsis haliana seeds. Phytochemistry. 17, 65-67