Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Effect of Low-molecularized Polymannuronate on Proliferation and DNA Synthesis of Human Colon Cancer Cells

저분자 polymannuronate의 인체 대장암세포 증식 및 DNA 합성 저해 효과

  • Kim In-Hye (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Nam Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyong National University)
  • 김인혜 (부경대학교 식품생명공학부) ;
  • 남택정 (부경대학교 식품생명공학부)
  • Published : 2005.12.01
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Abstract

This study investigated the proliferation and DNA synthesis inhibitory effect of concentrations ($0.01\%$, $0.1\%$, $0.25\%$, $0.5\%$) when added whole molecular-, 40 kDa-, or 10 kDa polymannuronate on human colon cancer cells, HT-29, DLD-1, and WiDr, in vitro. In order to determine the proliferation inhibitory effect of low-molecularized polymannuronate, the treatment of whole molecular-, 40 kDa-, 10 kDa-, polymannuronate ($0.25\%$) to the HT-29 cancer cells inhibited proliferation of cancer cells by $41\%$, $69.1\%$, and $75.6\%$, respectively. DLD-1 cancer cell was not relation of molecular weight and concentration. WiDr cancer cell depend on concentration without molecular weight. In addition, whole molecular-, 40 kDa-, 10 kDa poly mannuronate ($0.25\%$) significantly inhibited DNA synthesis of HT-29 cancer .cells by $78\%$, $58\%$, and $56\%$, respectively. And morphological changes not found under microscope by polymannuronate. Therefore polymannuronate would be helpful to colon cancer treatment as well as cancer prevention and this study would be the basic source for further research of polymannuronate.

본 연구는 whole molecular-, 40 kDa-, 10 kDa poly-mannuronate를 대장암세포에 첨가하였을 때 분자량과 농도 에 따른 항암효과를 세포수준에서 검토하고자 하였다. 3종류의 인체 대장암세포인 HT-29, DLD-1, WiDr의 증식 억제를 살펴본 결과, HT-29 세포는 모든 polymannuronate $0.25\%$의 농도에서 $50\%$의 증식 억제 효과를 보였으며 저분자화 될 수 록 증식 억제 효과가 증가되었다. DLD-1 세포는 저분자화 정도와 농도증가에 따른 유의적인 차이를 보이지 않았고, WiDr 세포는 농도증가에 따른 유의적인 효과는 있었으나, 분자량에 의한 차이는 보이지 않았다. $0.25\%$의 농도에서 $50\%$의 증식 억제 효과를 보인 HT-29 세포의 DNA 합성능을 살펴본 결과, 증식 억제 효과와 유사한 결과를 보였으며, 형태학적인 변화 또한 동일한 것으로 나타났다. 이상의 결과로부터 polymannuronate는 HT-29 대장암세포의 증식억제에 유의적인 효과가 있는 것으로 나타났다. 따라서 polymannuronate는 대장암의 예방뿐 아니라 그 치료에도 도움이 되며 본 연구가 polymannuronate의 효과에 대한 기초적인 자료가 될 것으로 사료된다.

Keywords

References

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