Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Use of comet assay as a bioassay in marine organisms exposed to genotoxicants

유전독성물질로 오염된 해양생물의 생물검정법으로서 comet assay 이용

  • Kim Gi-Beum (Marine Industry Institute, Gyeongsang National University) ;
  • An Joon-Gun (Marine Industry Institute, Gyeongsang National University) ;
  • Kim Jae-Won (Korea Inter-University Institute of Ocesn Science, Pukyong National University)
  • 김기범 (경상대학교 해양산업연구소) ;
  • 안준건 (경상대학교 해양산업연구소) ;
  • 김재원 (부경대학교 해양과학공동연구소)
  • Published : 2005.11.01
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Abstract

Using single cell gel electrophoresis, DNA single strand breaks were determined in various marine organisms. DNA damage on fish blood cells was detected to know whether there was a difference between Incheon, Pohang, Masan, and Tongyeong as a control site. Tongyeong showed the lowest DNA damage among the study areas. Mussels, transplanted to Masan Bay for one month, revealed high DNA damage at sites with high economical activity. In two weeks exposure of polychaete to Incheon sediments, higher DNA damage was detected in the sediment adjacent to Incheon harbor than open sea. These results suggested that the marine organism from the polluted area revealed a relatively high DNA damage. In addition, these areas might be contaminated with genotoxic compounds and comet assay was useful as a bioassay to detect DNA damage in marine organisms.

Keywords

References

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