Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Enhancement of Pendimethalin Degradation Activity in Bacillus sp. MS202 using Gamma Radiation

  • Lee Young-Keun (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute) ;
  • Chang Hwa-Hyoung (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute) ;
  • Lee Ho-Jin (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute) ;
  • Park Heesoon (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute) ;
  • Lee Kyung Hee (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute) ;
  • Joe Min-Ho (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
  • Published : 2005.12.01
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Abstract

To induce the enhanced mutants of dinitroaniline herbicide pendimethalin degrading bacterium, Bacillus sp. MS202 was irradiated with gamma radiation at the dose of $LD_{99}$ (3.35 kGy). Three enhanced mutants (MS202m7, MS202m14, MS202m18) were isolated from the candidates by the generation - isolation method. Clear zone formation and the GC analysis confirmed that the degrading activity of each enhanced mutant (MS202m7, MS202m14, MS202m18), the formation of pendimethalin metabolite, increased by $11\%,\;45\%,\;and\;32\%$ than a wild type, respectively. It suggested that these mutants induced by gamma radiation could be useful for the application of pesticide degradation.

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References

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