전자선 조사가 진공 포장된 계육 가슴살의 미생물학적 변화 및 품질에 미치는 영향

Effect of Electron Beam Irradiation on the Microbial Growth and Qualities of Chicken Breast

  • Ko, Jong-Kwan (Department of Food Science & Technology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Ma, Yu-Hyun (Department of Food Science & Technology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science & Technology, College of Agriculture & Life Sciences, Chungnam National University)
  • 발행 : 2005.06.30

초록

전자선 조사가 계육 가슴살의 미생물학적 변화와 품질에 미치는 영향을 조사하였다. 전자선 조사는 진공 포장하여 2-16 kGy의 선량으로 조사하였고, 조사 후 $4^{\circ}C$에서 7일 동안 저장하였다. 저장 중 전자선의 미생물에 대한 사멸효과를 확인하고, pH, 지방의 산패, 색도의 변화와 관능검사를 통해 전자선이 계육 가슴살의 품질에 미치는 영향을 분석하였다. 조사선량이 증가함에 따라 계육 가슴살에 존재하는 미생물 수가 유의적으로 감소함으로써 미생물학적 안전성의 가능성을 확인하였다. 저장 중 전자선 조사된 계육 가슴살의 pH를 확인한 결과, 조사선량에 따른 유의적인 차이는 보이지 않았다. TBARS 값의 경우, 조사선량과 저장기간의 증가에 따라 그 값이 유의적으로 증가함을 나타내었다. 조사된 시료에 대한 Hunter L, a, b value를 측정한 결과, a value가 조사선량과 저장기간의 증가에 따라 유의적으로 감소함을 나타내었고, L value와 b value의 경우 대조구와 비교하여 유의적 차이가 없었다. 관능검사의 경우, 조사직 후 조사선량에 따른 차이는 보이지 않았으나 저장기간이 증가함에 따라 전자선 조사된 시료가 관능적으로 우수함을 나타내었다. 본 연구 결과, 전자선 조사가 계육 가슴살의 미생물학적 안전성의 확보와 품질의 개선에 효과가 있음을 보여주었다.

Electron beam irradiation was applied to examine the microbial growth and qualities of vacuum-packaged chicken breasts. Chicken breast samples were irradiated at dose of 2, 4, 8, 12, and 16 kGy, respectively. After irradiation, chicken breast samples were individually vacuum-packaged and stored at $4^{\circ}C$. Microbiological change of irradiated vacuum-packaged chicken breasts showed that populations of total bacteria, yeast and mold, total coliform, and salmonella spp. in chicken breasts were significantly reduced with increasing irradiation dose. The pH values of vacuum-packaged chicken breasts were not significantly changed among treatments. Lipid oxidation measurements showed that TBARS values of vacuum-packaged chicken breasts increased with increase of irradiation dose, and gradually increased during storage. Color measurements showed that irradiation reduced Hunter a value of vacuum-packaged chicken breasts with increasing irradiation dose. However, Hunter L and b values of vacuum-packaged chicken breasts were not significantly altered among treatments. Sensory quality results of vacuum-packaged chicken breasts showed that there were no significant changes among the samples irradiated. These results indicate that irradiation can be used to improve the microbial safety and qualities of poultry products.

키워드

참고문헌

  1. Jimenez, S. M., Salsi, M. S., Tiburzi, M. C., Rafaghelli, Tessi, M. A. and Coutaz, V. R. (1997) Spoilage microflora in fresh chicken breast stored at $4^{circ}C$. J. Appl. Microbiol. 83, 613-618 https://doi.org/10.1046/j.1365-2672.1997.00276.x
  2. Bailey, J. S., Lyon, B. G., Lyon, C. E. and Windham, W. R. (2000) The microbiological profile of chilled and frozen chicken. J. Food Prot. 63, 1228-1230
  3. Dickens, J. A. and Whitemore, A. D. (1995) The effects of extended chilling times with acetic acid on the temperature and microbiological quality of processed poultry carcasses. Poult. Sci. 74, 1044
  4. Dorsa, W. J., Cutter, C. N., Siragusa, G. R. and Koohmaraie, M. (1995) Microbial decontamination of beef and sheep carcasses by steam, hot water spray washes, and a steamvaccum sanitizer. J. Food Prot. 59, 127
  5. CDC. (2004). Preliminary foodnet data on the incidence of infaction with pathogens transmitted commonly through food-Selected sites, United States, 2003. Morb. Mortal. Weekly Rep. 53, 338-343
  6. The Ministry of Health and Welfare in Korea. (1997) Food standard code. Seoul, Korea. 39, pp. 507-550
  7. Snyder, O. P. (1998) Menu management and purchasing: Food Safety Through Qualiy Assurance Management (ed. Hospitality Institute of Technology and Management). Chapter 6, Saint Paul, Minnesota, USA. pp. 11-13
  8. Kim, C. R., Kim, K. H., Moon, S. J., Kim, Y. J. and Lee, Y. K. (1998) Microbiological and physical quality of refrigerated chicken legs treated with acetic acid. Korean J. Food Sci. Biotechnol. 7, 13-17
  9. Jimenez-Villarreal, J. R., Pohlman, F. W., Johnson, Z. B. and Brown Jr, A. H. (2003) Effect of chlorine dioxide, cetylpyridinium chlorine, lactic acid and trisodium phosphate on physical and sensory properties of ground beef. Meat Sci. 65, 1055-1062 https://doi.org/10.1016/S0309-1740(02)00320-0
  10. Jimenez-Villarreal, J. R., Pohlman, F. W., Johnson, Z. B., Brown Jr, A. H. and Baublits, R. T. (2003) The impact of single antimicrobial intervention treatment with cetylpyridinium chloride, trisodium phosphate, chlorine dioxide or lactic acid on ground beef lipid, instrumental color and sensory characteristics.Meat Sci. 65, 977-984 https://doi.org/10.1016/S0309-1740(02)00315-7
  11. Boyette, M. D., Ritchie, D. F., Carballo, S. J., Blankenship, S. M. and Sanders, D. C. (1993) Chlorination and postharvest disease control. Hort. Technol. 3, 395-400
  12. Kraybill, H.F. (1978) Water Chlorination: Origin, classification and distribution of chemicals in drinking water with an assessment of their carcinogenic potential. Vol. 1, Jolly R. L. (ed). Ann Arbor Science, Ann Arbor, MI, USA. pp. 211-228
  13. Youm, H. J., Jang, J. W., Kim, K. R., Kim, H. J., Jeon, E. H., Park, E. K., Kim, M. R. and Song, K. B. (2004) Effect of chemical treatment with citric acid or ozonated water on microbial growth and polyphenol oxidase activity in lettuce and cabbage. J. Food Sci. Nutr. 9, 121-125
  14. Van Calenberg, S., Van haelewyn, G., Van Cleemput, O., Callens, F., Mondelaers, W. and Huyghebaert, A. (1998) Comparison of the effect of X-ray and electron beam irradiation on some selected spices. Lebensm-Wiss. u-Technol. 31, 252-258 https://doi.org/10.1006/fstl.1997.0352
  15. Jongen, Y., Abs, M., Genin, F., Nguyen, A., Capdevila, J. M. and Defrise, D. (1993) The Rhodotron, a new 10 MeV, 100 Kw, cw metric wave electron accelerator. Nucl. Instrum. Meth. 79, 865-870 https://doi.org/10.1016/0168-583X(93)95487-P
  16. Kwon, J. H., Byun, M. W. and Cho, H. O. (1992) Development of food irradiation technology and consumer attitude toward irradiation food in Korea. Radioisotopes 41, 654-662
  17. Ito, H. and Islam, S. (1994) Effect of does rate on inactivation of microorganisms in spices by electro-beams and gamma-rays irradiation. Radiat. Phys. Chem. 43, 545-550 https://doi.org/10.1016/0969-806X(94)90165-1
  18. Lee, M. K., Lee, M. H. and Kwon, J. H. (1998) Sterilizing effect of electron beam on ginseng powders. Korean J. Food Sci. Technol. 30, 1362-1366
  19. Lee, J. E. (1997) Effect of electron-beam irradiation on quality attributes of powdered red pepper and ginger. MS thesis, Kyungpook National University. Taegu, Korea
  20. Shamsuzzaman, K., Lucht, L. and Chuaquioffermanns, N. (1995) Effects of combined electron-beam irradiation and sousvide treatments on microbiological and other qualities of chicken breast meat. J. Food Prot. 58, 497
  21. Du, M., Nam, K. C. and Ahn, D. U. (2001) Cholesterol and lipid oxidation products in cooked meat as affected by rawmeat packaging and irradiation by cooked meat packaging and storage time. J. Food Sci. 66, 1396-1401 https://doi.org/10.1111/j.1365-2621.2001.tb15221.x
  22. Nam, K. C., Ahn, D. U. and Jo, C. (2001) Lipid oxidation, color, volatiles, and sensory characteristics of aerobically packaged and irradiated pork with different ultimate pH. J. Food Sci. 66, 1225-1229
  23. Bagorogoza, K., Bowers, J. and Okot-Kotber, M. (2001) The effect of irradiation and modified atmosphere packaging on the quality of intact chill-stored turkey breast. J. Food Sci. 66, 367-372 https://doi.org/10.1111/j.1365-2621.2001.tb11348.x
  24. Nanke, K. E., Sebranek, J. G. and Olson, D. G. (1999) Color characteristics of irradiated, aerobically packaged pork, beef, and turkey. J. Food Sci. 64, 272-278 https://doi.org/10.1111/j.1365-2621.1999.tb15881.x
  25. Nanke, K. E., Sebranek, J. G. and Olson, D. G. (1998) Color characteristics of irradiated vacuum-packaged pork, beef, and turkey. J. Food Sci. 63, 1001-1006
  26. Lee, J. E., Kwon, O. J. and Kwon, J. H. (2000) Effect of electron beam irradiation on microbiological and organoletic qualities of powdered red pepper and ginger. Korean J. Food Sci. Technol. 32, 380-386
  27. Lee, J. E., Lee, M. H. and Kwon, J. H. (2000) Effect of electron beam irradiation on physicochemical qualities of red pepper powder. Korean J. Food Sci. Technol. 32, 271-276
  28. Lee, M. K., Kwon, J. H. and Do, J. H. (1998) Effect of electron-beam irradiation on color and organoleptic qualities of ginseng. J. Ginseng Res. 22, 252-259
  29. Kwon, H., Lee, J. E., Kim, J. S. and Kwon, J. H. (2000) Effect of electron beam irradiation on the quality of Kochujang powder. J. Korean Soc. Food Sci. Nutr. 29, 655-662
  30. APHA. (2001) Compendium of methods for the microbiological examination of foods. American Public Health Association. Washington DC, USA
  31. IAEA. (1982) Training manual on food irradiation technology and techniques, technical report series. No 114, (2nd ed.), Vienna. pp. 43-60
  32. Ahn, D. U., Olson, D. C., Jo, C., Chen, X., Wu, C. and Lee, J. I. (1998) Effect of muscle type, packaging, and irradiation on lipid oxidation, volatile production, and color in raw pork patties. Meat Sci. 49, 29-39
  33. Kim S. K., Lee M. S., Lee K. T., Park S. K. and Song, K. B. (2004) Change in quality of pork and beef during storage and electronic nose analysis. Korean J. Food Pre. 11, 441-447
  34. Louise, M. F., Paul, E. C. and Alistair, S. G. (1997) The effect of electron beam irradiation, combined with acetic acid, on the survival and recovery of Escherichia coli and Lactobacillus curvatus. Int. J. Food Microbiol. 35, 259-265 https://doi.org/10.1016/S0168-1605(97)01251-8
  35. Clack, J. P. (2002) Processing papers and exhibits-electronic irradiation system. Food Technol. 56, 101-110
  36. Han, Y., Linton, R. H., Nielsen, S. S. and Nelson, P. E. (2000) Inactivation of Escherichia coli O157:H7 on surface-uninjured and -injured green pepper (Capsicum annuum L.) by chlorine dioxide gas as demonstrated by confocal laser scanning microscopy. Food Microbiol. 17, 643-655 https://doi.org/10.1006/fmic.2000.0357
  37. Du, J., Han, Y. and Linton, R. H. (2003) Efficacy of chlorine dioxide gas in reducing Escherichia coli O157:H7 on apple surfaces. Food Microbiol. 20, 583-591 https://doi.org/10.1016/S0740-0020(02)00129-6
  38. Fisher, T. L. and Golden, D. A. (1998) Fate of Escherichia coli O157:H7 in ground apples used in cider production. J. Food Prot. 61, 1372-1374
  39. Hayashi, T. (1991) In Food Irradiation: Comparatie effectiveness of gamma-rays and electron beams in food irradiation. Thorne, S. (ed.), Elsevier Applied Science. London and New York, pp. 169-206
  40. Ma, K. and Maxcy, R. B. (1981) Factors influencing radiation resistance of vegetative bacteria and spores associated with radappertization of meat. J. Food Sci. 46, 612-616 https://doi.org/10.1111/j.1365-2621.1981.tb04923.x
  41. Chung, S. S. and Han, Y. S. (2003) Consumer's recognition, nutrient composition, and safety evaluation of commercial Sunsik and Saengsik. Korean J. Food Culture 18, 235-243
  42. Holley, R. A., Gariepy, D., Delaquis, P., Doyon, G. and Gagnon, J. (1994) Static controlled atmosphere packaging retail ready pork. J. Food Sci. 59, 1296 https://doi.org/10.1111/j.1365-2621.1994.tb14699.x
  43. Heath, J. L., Owens, S. L., Tesch, S. and Hannah, K. W. (1990) Effect of high-energy electron irradiation of chicken on thiobarbituric acid values, shear values, odor, and cook yield. Poult. Sci. 69, 313-319
  44. O'Neill, E. (1993) The chemistry of muscle-based foods. Johnston, D. E., Knight, M. K. and Ledward, D. A. (ed.), Trends in Food Sci. Technol. 4, 349-350
  45. Luchsinger, S. E., Kropf, D. H., Garcia-Zepeda, C. M., Hunt, M. C., Marsden, J. L., Rubiocanas, E. J., Kastner, C. L., Kuecher, W. G. and Mata, T. (1996) Color and oxidative rancidity of gamma and electron beam irradiated boneless pork chops. J. Food Sci. 61, 1000-1005 https://doi.org/10.1111/j.1365-2621.1996.tb10920.x
  46. Lee, J. H., Sung, T. H., Lee, K. T. and Kim, M. R. (2004) Effect of gamma-irradiation on color, pungency, and volatiles of Korean red pepper powder. J. Food Sci. 69, 585-592 https://doi.org/10.1111/j.1365-2621.2004.tb09904.x
  47. Wetzel, K., Huebner, G. and Baer, M. (1985) International symposium on food irradiation processing. IAEA/FAO. Washington DC, USA. p. 13
  48. Cho, H. O., Kwon, J. H., Byun, M. W., Kim, Y. J. and Yang, J. S. (1986) Effect of ethylene oxide fumigation and gamma irradiation on the quality of ground red and black peppers. Korean J. Food Sci. Technol. 18, 294-300