Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of Sterols from the Methanol Extracts of Cymbidium goeringii REICHB. fil

춘란(Cymbidiym goeringii REICHB. fil)의 메탄올 추출물로부터 Sterol 화합물의 분리

  • Lee, Jin-Hee (Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Kim, Dong-Hyun (Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yang, Hye-Joung (Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Bang, Sung-Hoon (Horticulture, KyungHee University) ;
  • Chung, In-Sik (Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, KungHee University) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Park, Mi-Hyun (Erom Life Co. Ltd.) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center)
  • 이진희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김동현 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 방면호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 양혜정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 방성훈 (경희대학교 원예학과) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 권병목 (한국생명공학연구원) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 김대근 (우석대학교 약학대학) ;
  • 박미현 ((주)이롬라이프) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2005.09.30
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Abstract

Cymbidium goeringii REICHB. fil was extracted with 80% MeOH and solvent-fractionated with EtOAc, n-BuOH and $H_2O$, successively. From EtOAc fraction, three sterols were isolated on repeated silica gel and ODS column chromatographies. The chemical structures were determined as ${\beta}-sitosterol$ (1), daucosterol (2) and ergosterol peroxide (3) by NMR, MS and IR, which is the first to be isolated from Cymbidium goeringii REICHB. fil.

춘란을 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. EtOAc 분획에 대하여 column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 ${\beta}-sitosterol$, daucosterol, ergosterol peroxide로 구조를 결정하였다. 이 화합물들은 춘란에서 이번에 처음 분리, 보고되었다.

Keywords

References

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