Applied Biological Chemistry

  • 제48권4호
  • /
  • Pages.431-434
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  • 2005
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

HPLC에 의한 산지별 한국산 석류과피 중 항산화화합물의 함량분석

Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site

  • 곽혜민 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 정현희 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 송방호 (경북대학교 사범대학 생물교육과) ;
  • 김종국 (경북대학교 생명공학부) ;
  • 이진만 (경북과학대학) ;
  • 허종문 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 송경식 (경북대학교 농업생명과학대학 응용생물화학부)
  • Kwak, Hye-Min (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jeong, Hyun-Hee (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Bang-Ho (College of Teachers, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences & Biotechnology, Kyungpook National University) ;
  • Lee, Jin-Man (Department of Herb & Food Science, Kyungpook college of Sciences) ;
  • Hur, Jong-Moon (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
  • 발행 : 2005.12.31
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초록

한국산 석류 과피 중 주요 성분으로 함유되어 있는 ellagic acid와 punicalagin에 대한 HPLC 정량법을 확립하였다. 확립된 방법은 다음과 같다. 즉 column은 Agilent Zorbax Eclipse XDB-C18($4.6{\times}150mm,\;5{\mu}m$), 이동상은 1% formic acid를 포함하는 순수(A)와 1% formic acid를 함유하는 MeCN(B)을 이용하여(A) 용매가 5%에서 50분 후 100%가 되도록 농도기울기를 주어 용출하며, 이 때 유속은 0.8 ml/min., 검출은 UV 254 nm이다. 석류 과피 중 ellagic acid와 punicalagin 함량을 분석하기 위한 최적 전처리 추출조건을 확립한 결과, 100 ml의 95% ethanol로 5 g의 석류 과피를 3시간 환류추출한 경우 상기 항산화 화합물의 추출효과가 가장 좋았다. 확립된 최적 분석 조건을 이용하여 재배지가 다른 국내산 석류 과피 5종 중 함량을 조사한 결과 ellagic acid의 함량은 해평산이 $15.27{\mu}g/mg$으로 가장 높았으며, punicalagin의 함량은 장성산이 $16.21{\mu}g/mg$으로 가장 높았다. 한편 이들의함량은 산지별로 매우 큰 차이를 보여 한국산 석류피를 건강기능식품 등의 원료로 이용할 경우 산지의 선택이 매우 중요할 수 있음을 시사하였다.

The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

키워드

참고문헌

  1. Proctor, P. H. (1992) Free radicals and human disease. In Handbook of free radicals and antioxidants in medicine. Vol. I, CRC Press, Boca Raton, FL. pp. 17
  2. Zwart, L. L., Meerman, J. H. N., Commander, N. M. and Vemeulen, N. P. E. (1996) Biomarkers of free radical damage applications in experimental animals and humans. Free Radical Biol. Med. 26, 202-226 https://doi.org/10.1016/S0891-5849(98)00196-8
  3. Nakayama, T., Niimi, T., Osawa, T. and Kawakishi, S. (1992) The protective role of polyphenols in cytotoxicity of hydrogen peroxide. Mutat. Res. 281, 77-80 https://doi.org/10.1016/0165-7992(92)90039-K
  4. Bengendi, L., Benes, L., Durackova, Z. and Ferencik, M. (1999) Chemistry, physiology and pathology of free radicals. Life Sci. 65, 1864-1874
  5. McCord, J. M. and Fridovich, I. (1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049-6055
  6. Lodovici, M., Guglielmi, F., Meoni, M. and Dolara, P. (2001) Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 39, 1205-1210 https://doi.org/10.1016/S0278-6915(01)00067-9
  7. Halliwell, B., Murcia, M. A., Chirico, S. and Aruoma, O. I. (1995) Free radicals and antioxidants in food and vivo: what they do and how they work. Crit. Rev. Food Sci. Nutr. 35, 7-20 https://doi.org/10.1080/10408399509527682
  8. Meydani, S. N., Wu, D., Santos, M. S. and Hayek, M. (1995) Antioxidants and immune response in aged persons: overview of present evidence. Am. J. Clin. Nutr. 62, 14625-14765
  9. Hamilton, R. J., Kalu, C., Prisk, E., Padley, F. B. and Pierce, H. (1997) Chemistry of free radicals in lipids. Food Chem. 60, 193-199 https://doi.org/10.1016/S0308-8146(96)00351-2
  10. Steinberg, D. (1991) Antioxidants and atherosclerosis: a current assessment. Circulation 84, 1420-1425
  11. Ames, B. M., Shigena, M. K., Hagen, T. M. (1993) Oxidants, antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci. 90, 7915-7922 https://doi.org/10.1073/pnas.90.17.7915
  12. Gackowski, D., Kruszewski, M., Jawien, A., Ciecierski, M. and Olinski, R. (2001) Further evidence that oxidant stress may be a risk fator responsible for the development of atherosclerosis. Free Radical Biol. Med. 31, 542-547 https://doi.org/10.1016/S0891-5849(01)00614-1
  13. Fuhrman, B., Volkova, N., Rosenblat, M. and Aviram, M. (2000) Lycopene synergistically inhibits LDL oxidation in combination with vitamin E, glabridin, rosmarinic acid, carnisic acid, or galic. Antioxidants Redox Signaling 2, 491-506 https://doi.org/10.1089/15230860050192279
  14. Oyangui, Y. (1989) SOD and active oxygen modulators. Nihon Igakukan, Tokyo, pp. 17
  15. Chang, S. T., Wu, J. H., Wang, S. Y., Kang, P. L., Yang, N. S. and Shyur, L. F. (2001) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agric. Food Chem. 49, 3420-3424 https://doi.org/10.1021/jf0100907
  16. Lu, Y., Foo, Y. (2001) Antioxidant activities of polyphenols from sage (Salvia ofjicinals). Food Chem. 75, 197-202 https://doi.org/10.1016/S0308-8146(01)00198-4
  17. Wang, S. Y. and Jiao, H. (2000) Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydrogen peroxide, hydroxly radicals, and singlet oxygen. J. Agric. Food Chem. 48, 5677-5684 https://doi.org/10.1021/jf000766i
  18. Burda, S. and Oleszaw, W. (2001) Antioxidant and antiradical activities of flavonids. J. Agric. Food Chem. 49, 2774-2779 https://doi.org/10.1021/jf001413m
  19. Hollman, P. C. H. and Katan, M. B. (1999) Dietary flavonoids: intake, health effects and bioavailalility. Food Chem. Toxicol. 73, 937-942
  20. Lodovici, M., Guglielmi, F., Meoni, M. and Dolara, P. (2001) Effect of natural phenolic acids on DNA oxidation in vitro. Food Chem. Toxicol. 39, 1205-1210 https://doi.org/10.1016/S0278-6915(01)00067-9
  21. Singh, R., Murthy, K. C. and Jayaprakasha, G. (2002) Studies on the antioxidant activity of pomegranate (P. granatum) peel and seed extract using in vitro models. Agric. Food Chem. 50, 81-86 https://doi.org/10.1021/jf010865b
  22. Schubert, S. Y., Lansky, E. P. and Neeman, I. (1999) Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J. Ethnopharmacol. 66, 11-17 https://doi.org/10.1016/S0378-8741(98)00222-0
  23. Noda, Y., Kaneyuki, T., Mori, A and Packer, L. Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelagonidin. J. Agric. Food Chem. 50, 166-171 https://doi.org/10.1021/jf0108765
  24. Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D. and Fuhrman, B. (2000) Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am. J. Clin Nutr. 71, 1062-1076
  25. Gil, M. I., Tomas-Barberan, F. A, Hess-Pierce, B., Holcroft, D. M. and Kader, A. A. (2000) Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem. 48, 4581-4589 https://doi.org/10.1021/jf000404a
  26. Koh, J.-H., Hwang, M.-O., Moon, J.-S., Hwang, S.-Y. and Son, J.- Y. (2005) Antioxidative and antimicrobial activities of pomegranate seed extract. Kor. J. Food Cookery Sci. 21, 171-179
  27. Shim, S.-M., Choi, S.-W. and Bae, S.-J. (2001) Effects of Punica granatum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J. Kor. Soc. Food Sci. Nutr. 30, 80-85
  28. Poyrazogiu, E., Gokmen, V. and Atrik, N. (2002) Organic Acids and Phenolic Compound in Pomegranates (Punica granatum L.) Grown in Turkey. J. Food Composit. Anal. 15, 567-575
  29. Kwak, H.-M., Jeon, S.-Y., Sohng, B.-H., Kim, J.-K, Lee, J.-M., Lee, K-B., Jeong, H.-H., Hur, J.-M. (2005) $\beta$-Secretase (BACEl) inhibitors from pomegranate peel. Arch. Pharm. Res. submitted