Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Quantitative Analysis of Antioxidants in Korean Pomegranate Husk (Granati pericarpium) Cultivated in Different Site

HPLC에 의한 산지별 한국산 석류과피 중 항산화화합물의 함량분석

  • Kwak, Hye-Min (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jeong, Hyun-Hee (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Bang-Ho (College of Teachers, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences & Biotechnology, Kyungpook National University) ;
  • Lee, Jin-Man (Department of Herb & Food Science, Kyungpook college of Sciences) ;
  • Hur, Jong-Moon (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Song, Kyung-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
  • 곽혜민 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 정현희 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 송방호 (경북대학교 사범대학 생물교육과) ;
  • 김종국 (경북대학교 생명공학부) ;
  • 이진만 (경북과학대학) ;
  • 허종문 (경북대학교 농업생명과학대학 응용생물화학부) ;
  • 송경식 (경북대학교 농업생명과학대학 응용생물화학부)
  • Published : 2005.12.31
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Abstract

The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

한국산 석류 과피 중 주요 성분으로 함유되어 있는 ellagic acid와 punicalagin에 대한 HPLC 정량법을 확립하였다. 확립된 방법은 다음과 같다. 즉 column은 Agilent Zorbax Eclipse XDB-C18($4.6{\times}150mm,\;5{\mu}m$), 이동상은 1% formic acid를 포함하는 순수(A)와 1% formic acid를 함유하는 MeCN(B)을 이용하여(A) 용매가 5%에서 50분 후 100%가 되도록 농도기울기를 주어 용출하며, 이 때 유속은 0.8 ml/min., 검출은 UV 254 nm이다. 석류 과피 중 ellagic acid와 punicalagin 함량을 분석하기 위한 최적 전처리 추출조건을 확립한 결과, 100 ml의 95% ethanol로 5 g의 석류 과피를 3시간 환류추출한 경우 상기 항산화 화합물의 추출효과가 가장 좋았다. 확립된 최적 분석 조건을 이용하여 재배지가 다른 국내산 석류 과피 5종 중 함량을 조사한 결과 ellagic acid의 함량은 해평산이 $15.27{\mu}g/mg$으로 가장 높았으며, punicalagin의 함량은 장성산이 $16.21{\mu}g/mg$으로 가장 높았다. 한편 이들의함량은 산지별로 매우 큰 차이를 보여 한국산 석류피를 건강기능식품 등의 원료로 이용할 경우 산지의 선택이 매우 중요할 수 있음을 시사하였다.

Keywords

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