Korean Journal of Clinical Pharmacy (한국임상약학회지)
- Volume 15 Issue 1
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- Pages.46-49
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- 2005
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- 1226-6051(pISSN)
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- 2508-786X(eISSN)
Validation of a HPLC Method for Determination of Pentoxifylline in Human Serum and Its Application to Single-dose Pharmacokinetics
인체혈청 중 펜톡시필린의 정량을 위한 HPLC 분석법의 점증 및 단일용량 투여에 의한 약물동태
- Sung, Min Kyung (College of Pharmacy, Ewha Womans University) ;
- Park, Hyo Min (College of Pharmacy, Ewha Womans University) ;
- Kim, Na Hyung (College of Pharmacy, Ewha Womans University) ;
- Chung, Soo Yeon (College of Pharmacy, Ewha Womans University) ;
- Go, Eun Jung (College of Pharmacy, Ewha Womans University) ;
- Lee, Hwa Jeong (College of Pharmacy, Ewha Womans University)
- 성민경 (이화여자대학교 약학대학) ;
- 박효민 (이화여자대학교 약학대학) ;
- 김나형 (이화여자대학교 약학대학) ;
- 정수연 (이화여자대학교 약학대학) ;
- 고은정 (이화여자대학교 약학대학) ;
- 이화정 (이화여자대학교 약학대학)
- Published : 2005.06.01
Abstract
A simple HPLC method was employed for the determination of pentoxifylline in human serum. After addition of internal standard (IS, 50 uL of 3 ug/mL chloramphenicol methanol solution) into the serum sample, the drug and IS were extracted by dichloromethane. Following a 1-min vortex-mixing and a 15-min centrifugation at 3500 게m, the organic phase was transferred and evaporated to dryness under a vacuum. The residue was reconstituted with 120