Effects of Proton Beam Irradiation on Germination and Growth of Tobacco and Rice Plants

담배와 벼의 발아와 생장에 대한 Proton 빔조사의 영향

  • Lyu Jae-Il (Division of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Sarantuya Gendaram (Division of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Chai Jong-Seo (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Kim Jae-Hong (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Yang Tae-Gun (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Lee Min-Yong (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Yang Deok-Chun (College of Life Science & Center for Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Bae Chang-Hyu (Division of Plant Production Sciences, College of Agriculture and Life Sciences, Sunchon National University)
  • 류재일 (순천대학교 농업생명과학대학 식물생산과학부) ;
  • 사란투야 젠다람 (순천대학교 농업생명과학대학 식물생산과학부) ;
  • 채종서 (한국 원자력의학원) ;
  • 김재홍 (한국 원자력의학원) ;
  • 양태건 (한국 원자력의학원) ;
  • 이민용 (한국 원자력의학원) ;
  • 양덕춘 (경희대학교 한방재료가공센터) ;
  • 배창휴 (순천대학교 농업생명과학대학 식물생산과학부)
  • Published : 2005.10.01

Abstract

Effects of proton beam irradiation on seed germination and growth pattern of tobacco (Nicotiana tabacum L. cv. BY-4; N. plumbaginifolia) and rice (Oryasativa L.) plants were estimated to develop the efficient conditions of irradiation. Seed germination rate was decreased by increasing the proton beam the current and the beam irradiation time in both tobacco and rice seeds. The beam irradiation conditions showing $50\%$ germination were over 60 sec at 10 nA, approximately 5 sec at 100 nA and at 500 nA beam current in tobacco seeds. And the conditions of $50\%$ germination were 60 sec at 10 nA, and 100 nA and 30 sec at 500nA in rice (cv. Dongjin 1) seeds. The growth of irradiated plants was decreased, but significant difference in morphological changes was not observed by the proton beam treatment. The proton beam is able to use as a mutagen, but some of the factors including beam size and beam detector-system must be established for efficient usage of the beam.

Keywords

References

  1. Abe, T., C.H. Bae, T. Ozaki, J.M. Wang and S. Yoshida. 2000. Stress-tolerant mutants induced by heavy-ion beams. Gamma Field Symposia 39:45-56
  2. Bae, C.-H., T. Abe, T. Matsuyama, N. Fukunish, N. Nagata, T. Nakano, Y. Kaneko, K. Miyoshi, H. Matsushima and S Yoshida. 2001. Regulation of chloroplast gene expression is in ali, a novel tobacco albino mutant. Annals of Botany 88:545-553 https://doi.org/10.1006/anbo.2001.1495
  3. Bae, C.-H., T. Abe, N. Nagata, N. Fukunish, T. Matsuyama, T. Nakano and S. Yoshida. 2000. Characterization of a periclinal chimera variegated tobacco (Nicotiana tabacum L.). Plant Science 151 :93-101 https://doi.org/10.1016/S0168-9452(99)00205-8
  4. Chen, Y., B. Jiang, Y. Chen, X. Ding, X. Liu, C. Chen, X. Guo and G. Yin. 1998. Formation of plasmid DNA strand breaks induced by low-energy ion beam: induction of nuclear stopping effects. Radiat. Environ. Biophys. 37:101-106 https://doi.org/10.1007/s004110050101
  5. Hase, Y., K. Shimono, M. Inoue, A. Tanaka and H. Watanabe. 1999. Biological effects of ion beams in Nicotiana tabacum L. Radiat. Environ. Biophys. 38:111-115 https://doi.org/10.1007/s004110050146
  6. Li, X. and Y. Zheng. 2002. Reverse genetics by fast neutron mutagenesis in higher plants. Funct. Integr. Genomics 2:254-258 https://doi.org/10.1007/s10142-002-0076-0
  7. Tanaka, A., N. Shikazono, Y. Yokota, H. Watanabe and S. Tano. 1997. Effects of heavy ions on the germination and survival of Arabidopsis thaliana. Int. J. Radiat. BioI. 72:121-127 https://doi.org/10.1080/095530097143608
  8. Vazquez-Tello, A., T. Uozumi, M. Hidaka, Y. Kobayashi and H. Watanabe. 1996. Effect $of ^ {12}C^{+5} $ ion beam irradiation on cell viability and plant regeneration in call us, protoplasts and cell suspensions of Lavatera thuringiaca. Plant Cell Rep. 16:46-49
  9. Wu, L. and Z. Yu. 2001. Radiobiological effects of a low-energy ion beam on wheat. Radiat. Environ. Biophys. 40:53-57 https://doi.org/10.1007/s004110000078
  10. 膏藤稔,小森治責,板本j告,畑下昌範,高城啓一,阿部知子,吉田茂男.2004.イオンビームを用いた突 然燮異育種.加速器利用グループ植物照射利用 者舎報告書 2003 p13
  11. 배창휴, T. Abe, 김동철, 이영일, 정재성, 민경수, 이 효연 .2000. 중이온 beam 조사가 담배의 약과 캘러스 및 종자에 미치는 영향. 한국식물조직배양학회지 27: 109-115
  12. 배창휴, T. Abe, 민경수, 김동철, 정재성, 이춘환, 임 용표, 이효연. 1998. 중이온 beam 조사에 의한 돌연변이 유도와 내염성 식물의 선발. 한국식물조직배양학회지 25(2):89-94