Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Characteristics of Tobacco and Rice Plants Irradiated with Neutron Beam

Neutron 빔조사 담배 및 벼식물체의 특성

  • Chai Jong-Seo (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Kim Jae-Hong (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Yang Tae-Gun (Cyclotron Application Lab., Korea Institute of Radiological & Medical Sciences (KIRAMS)) ;
  • Lyu Jae-Il (Division of Plant Production Sciences , College of Agriculture and Life Sciences, Sunchon National University) ;
  • Lee Hyo-Yeon (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University) ;
  • Yang Deok-Chun (College of Life Science & Center for Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Bae Chang-Hyu (Division of Plant Production Sciences , College of Agriculture and Life Sciences, Sunchon National University)
  • 채종서 (한국 원자력의학원) ;
  • 김재홍 (한국 원자력의학원) ;
  • 양태건 (한국 원자력의학원) ;
  • 류재일 (순천대학교 농업생명과학대학 식물생산과학부) ;
  • 이효연 (제주대학교 생명자원과학대학 생명공학부) ;
  • 양덕춘 (경희대학교 한방재료가공센터) ;
  • 배창휴 (순천대학교 농업생명과학대학 식물생산과학부)
  • Published : 2005.10.01
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Abstract

Effects of neutron beam irradiation on seed germination, growth and RAPD pattern of tobacco (Nicotiana tabacum L. cv.; N. plumbaginifolia) and rice (Orya sativa L. cv.) plants were estimated. Seed germination rate was not significantly changed by the neutron beam treatment in both tobacco and rice seeds. And there was no significant differance in growth of the plants by the neutron beam treatment. Interestingly, however, some of morphological changes, including leaf shape (about $36\%$), stem color and leaf color were observed in neutron beam treated tobacco plants. In addition, abnormal flower in petal was observed in the neutron beam treated plant. This results indicate that neutron beam is able to use as an effective mutagen in plant mutations. Scorable products from 20 primers were obtained by RAPD analysis in the leaves of the beam irradiated tobacco plants and most of the plants showed the similar band patterns.

재배종담배(Nicotiana tabacum L. cv.), 야생종담배 (N. plumbaginifolia)와 벼종자(Orya sativa L, cv.)에 neutron빔을 각각 조사(irradiation)하여 발아, 식물체의 생장과 DNA의 변이에 미치는 영향을 검토하였다. 담배종자와 벼종자에 90, 180, 270, 360, 450, 540 Gy까지 조사로 발아율은 크게 감소하지 않았고, 생장에 있어 유의적인 차이는 없었다. 그러나 담배식물체에서 총 200개체 중 71개체(약 $36\%$)가 잎에서 형태 이상을 나타내었다. 또한 줄기색 변이체, 엽색변이체, 화형변이체를 유도하였다. 이 결과는 neutron빔이 유용한 돌연변이원으로서 가능성이 있음을 시사해 준다. Neutron빔을 조사한 후 생장한 담배식물체의 잎에 대하여 총 34개의 primer를 이용하여 RAPD 분석한 결과 20개의 primer에서 총 104개의 DNA 단편이 증폭되었고, 중성자 빔조사처리구에서만 출현하는 DNA 단편은 나타나지 않았다.

Keywords

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