Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of Cryopreservation of Sperm and Embryos on fertilization, Development and Pregnancy in Int Application

정자와 수정란의 동결이 ICSI 시술에서 수정, 발생 및 임신에 미치는 영향

  • Min Sung-Hun (Department of Animal Science and Biotechnology, kyungpook National University) ;
  • Park Yong-Soo (Kyoungbuk Livestock Research Institute) ;
  • Park Young-Sok (Department of Animal Science and Biotechnology, kyungpook National University)
  • 민성훈 (경북대학교 농업생명과학대학 동물공학과) ;
  • 박용수 (경상북도축산기술연구소) ;
  • 박영식 (경북대학교 농업생명과학대학 동물공학과)
  • Published : 2005.09.01
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Abstract

The cryopreservation of germ cells, sperm and embryos, has been largely used to increase the effect of artificial reproductive techniques for human infertility, but the efficiency of germ cell cryopreservation has been conkoversial till now. Thus, the effect of the cryopreservation of human sperm used for ICSI and the effect of the cryopreservation of embryos produced by ICSI on fertilizatiof development and pregnancy were investigated. Sperm freezing did not affect fertilizatiort development and pregnancy rates. Also, there was no significant difference between ejaculated and testicular sperm in ferclizatiort development and pregnancy. Embryo freezing methods, slow freezing and vitrificatior did not differ each other in viability and pregnncy rates. However, ICSI embryo freezing significantly decreased pregnancy rate compared to fresh embryos freezing (p<0.05). In conclusiof this result suggested that cryopreservation of sperm for ICSI did not affect on the resulted embryo development and pregnancy, but ICSI embryo cryopreservation would significantly inhibit pregnancy.

인간의 불임을 극복하기 위한 번식공학 기술의 효율성을 증가시키기 위해 성세포의 동결이 널리 수행되고 있으나 동결 기술의 효율성에 있어서 논란의 여지가 있다. 본 연구에서는 체외수정란을 생산하기 위한 난자세포질내 정자미세주입(ICSI) 시술에 사용되는 정자와 이들 기술을 이용 생산한 체외수정란의 동결이 배 발생 및 임신에 미치는 효과를 조사하였다. ICSI방법으로 체외수정란을 생산하는 경우 정자의 동결이 체외수정, 발생 및 임신에 영향을 미치지 않았으며, 특히 동결융해한 사출 및 정소정자에 의한 체외수정율과 발생율 및 임신율도 차이가 없었다. 한편 체외수정란을 동결하는 경우 완만동결과 초자화동결에 의한 체외수정란의 생존율과 임신율은 차이가 없었으나, 동결수정란은 신선수정란에 비하여 임신율이 유의하게 낮았다(p<0.05). 결론적으로 ICSI에 사용되는 정자와 달리 ICSI에 의해 생산된 수정란을 동결하는 경우 임신율을 저하시킬 수 있다.

Keywords

References

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