Biomedical Science Letters (대한의생명과학회지)
- Volume 11 Issue 3
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- Pages.355-363
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- 2005
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Protective Effect of Vascular Endothelial Growth Factor on Focal Cerebral Ischemia in Rats
- Noh, Yong-Rae (Department of Neurosurgery, College of Medicine, Pusan National University) ;
- Lee, Won-Suk (Department of Pharmacology, College of Medicine, Pusan National University) ;
- Choi, Chang-Hwa (Department of Neurosurgery, College of Medicine, Pusan National University)
- Published : 2005.09.01
Abstract
This study aimed to investigate the cerebroprotective effect of vascular endothelial growth factor (VEGF) on permanent focal cerebral ischemia in Sprague-Dawley rats. Right middle cerebral artery (MCA) was occluded for 6 and 24 hours by an intraluminal monofilament technique. An open cranial window was made on the right parietal bone for determination of continuous changes in regional cerebral blood flow (rCBF) by laser-Doppler flowmetry. The infarct size was morphometrically determined using the 2,3,5-triphenyltetrazolium chloride technique. Brain edema was determined by measuring brain water content. In normal rats, rCBF was significantly increased by intravenous infusion of VEGF for 10 minutes. The VEGF-induced increase in rCBF was significantly inhibited by pretreatment with suramin, a heparin-binding growth factor inhibitor as well as
Keywords
- Vscular endothelial growth factor;
- Focal cerebral ischemia;
- Regional cerebral blood flow;
- Cerebral infarct;
- Brain edema