Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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A Biosensor for Diagnosis of Swine Sarcoptic Mange

돼지 옴 감염증 진단을 위한 바이오센서 연구

  • Cho H. K. (Dept. of Biosystems Engineering, Chungbuk National University) ;
  • Jee C. H. (Dept. of Veterinary Medicine, Chungbuk National University)
  • Published : 2005.10.01
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Abstract

In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.

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References

  1. Cai, J. J., David, K. S., Xuhua, X. and Yuen, Kwokyung. 2005. Software MBET Toolbox: a Matlab toolbox for sequence data analysis in molecular biology and evolution. BMC Bioinfromatics, 6:64. or http//www.biomedcentral.com/1471-2105/6/64
  2. Davies, P. R., Bahnson, P. B., Cgass, J. J., Marsh, W. E. Garcia, R., Melancon, J. and Dial, G. D. 1966. Evaluation of the monitoring of popular dermatitis lesions in slaughtered swine to assess sarcoptic mite infestation. Vet Parasitol, 62: 143-153 https://doi.org/10.1016/0304-4017(95)00853-5
  3. Gershoni, J. M. and Palade, G. E. 1983. Protein blotting: principles and application. Anal Biochem, 131:1-15 https://doi.org/10.1016/0003-2697(83)90128-8
  4. Gonzaleze, J. M., Gonzaleze, A. J. and Caabeiro, F. R. 1998. Purification of Trichinella spiralis tubulin : Comparison of several analytic procedures. Vet. Parasitol, 77:115-121 https://doi.org/10.1016/S0304-4017(98)00098-3
  5. Lower, S., Kimberly, L. M., Medleau, Hnilica, K. and Bigler, B. 2001. Veterinary Dermatology, 12, 315-320 https://doi.org/10.1046/j.0959-4493.2001.00265.x
  6. Melancon, J. J. 1998. Sarcoptic mnage in swine: current prevalence. Compend Cont Educ Pract Vet, 20:87-95
  7. Smets, K. J. and Vercruysse, J. 2000. Evaluation of different methods for the diagnosis of scabies in swine. Vet Parasitol, 90:137-145 https://doi.org/10.1016/S0304-4017(00)00222-3
  8. Wooten, E. L. and Gaafer, S. M. 1984. Detection of serum antibodies to sarcoptic mange mite antigens by the passive hemagglutination assay in pigs infested with Sarcoptes scabiei var. suis. Vet Parasitol, 15:317-323 https://doi.org/10.1016/0304-4017(84)90084-0
  9. 김재원, 지차호. 2004. 연속적 용출법을 이용한 개옴과 집먼지 진드기의 공통항원의 분리. J. of Vetinary medicine and Biotechnology, (5)1:69-78
  10. 임종근, 조한근. 2005. 살충제 Imidacloprid 잔류물의 신속한 측정을 위한 생물반응 및 계측제어 시스템 개발. 바이오시스템 공학, (30)2:114-120
  11. 지차호, 이삼선, 장래훈. 2001. ELISA를 이용한 돼지 옴 감염증의 진단. 대한수의학회지, (41)4:565-570