한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제38권5호
  • /
  • Pages.304-308
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  • 2005
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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Diethylhexyl Phthalate에 노출된 동자개, Pseudobagrus fulvidraco의 항산화 효소활성의 변동

Changes of Antioxidant Enzyme Activity in Bagrid Catfish, Pseudobagrus fulvidraco Exposed to Diethylhexyl Phthalate

  • 금유화 (부경대학교 수산생명의학과) ;
  • 지정훈 (부경대학교 수산과학연구소) ;
  • 구자근 (인천수산종묘배양연구소) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • KEUM Yoo-Hwa (Department of Aquatic Life Medicine, Pukyong National University) ;
  • JEE Jung-Hoon (Institute of Fisheries Sciences, Pukyong National University) ;
  • KOO Ja-Geun (Incheon Fisheries Hatchery Research Institute) ;
  • KANG Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • 발행 : 2005.10.01
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초록

The effects of diethylhexyl phthalate (DEHP) on various oxidative stress responses in liver, kidney and gill tissues of freshwater bagrid catfish Pseudobagrus fulvidraco were investigated under laboratory conditions. Bagrid catfish were intraperitoneally injected with sunflower seed oil containing nominal concentrations of 0, 300 or 900mg DEHP per kilogram of body weight for 3 days and the effects after last injection were assessed in liver, kidney and gill tissues of the exposed organisms. The oxidative stress responses of fish were evaluated by analyzing the level of glutathione (GSH), as well as the activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR). After exposure to the DEHP, there were significant decrease in GR, GPx activity and GSH content in liver of fish exposed to 900 mg DEHP per kilogram of body weight compared to the control group. Compared with the control group, significant decreases in renal GPx and GR activity were observed in the DEHP treatment groups (900 mg $kg^{-1}$ bw). However, no significant difference was observed in any oxidative stress responses in gills between the DEHP-treated and the untreated group of fish. The findings of the present investigation show that DEHP induce oxidative stress and the liver was the most affected organ followed by the kidney and gills. Furthermore, the changes of GPx and GR activities may be important indicators of oxidative stress responses but additional study is required to confirm the oxidative stress of DEHP.

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