Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Study on the Role of Estrogen Receptor-Alpha in Yak-Kong and Soybean Induced Proliferation of MG-63 Human Osteoblastie Cells

약콩 (Rhynchosia volubilis: 서목태) 및 대두 처리에 의한 MG-63 조골세포 증식 증가에서 ER$\alpha$의 역할에 대한 연구

  • Um, So-Jung (Department of Medical Nutrition, Graduate School of East-West Medical Science) ;
  • Kang, In-Sook (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Cho, Yun-Hi (Department of Medical Nutrition, Graduate School of East-West Medical Science)
  • 엄소정 (경희대학교 동서의학대학원 의학영양학과) ;
  • 강인숙 (경희대학교 의과대학 생화학교실) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Phytoestrogens, especially Yak-kong or soybean-derived isoflavones have been traditionally used as a supplement of estrogen for preventing postmenopausal osteoporosis in oriental folk medicine. In a previous study, we demonstrated that as Yak-kong and soybean increased MG-63 human osteoblastic cell proliferation, the expression of estrogen receptor $\alpha\;and\;beta\;(ER\;\alpha:\;ER\;\beta$) both were increased. However, the increased level of ER $\alpha$ is much higher than that of ER$\beta$. To determine whether the altered level of ER $\alpha$ expression affects Yak-kong or soybean induced MG-63 cell proliferation, we established cell lines stably expressing either ER $\alpha$ or antisense ER $\alpha$ RNAs. Increased expression of ER a in MG- 63 cells (ER $\alpha$-MG63) enhanced Yak-kong or soybean induced proliferation which paralleled with the enhanced expression of IGF-I. Inhibition of ER $\alpha$ expression by antisense $ER\;\alpha\;RNAs\;(As-ER\;\alpha-MG63$) caused these cells to insensitize Yak- kong or soybean induced proliferation and IGF-I expression. Furthermore, the comparable effects between Yak-kong and the combined treatment of genistein and daidzein at $0.5\;{\times}\;10^{-8}M$, which is a concentration of these two isoflavones similar to Yak-kong at 0.001 mg/ml, on cell proliferation and IGF-I expression in $ER\;\alpha-MG63\;or\;As-ER\;\alpha-MG63$ cells demonstrate that ER $\alpha$ plays an important, active role in MG-63 cell proliferation induced by phytoestrogens, especially Yak-kong or soybean derived isoflavones.

Keywords

References

  1. Kim KS, Min BK, Lee SP, Kim IS, Kim HY, Sim JR. Evaluation of biochemical markers of bone turnover in postmenopausal osteoporotic women to Alendronate treatment J Korean Soc Merwpause 6 (1): 36-42, 2000
  2. Kim KS. Osteoporosis. The Women's News. Seoul, pp10-11 , 1998
  3. Rogers J. Estrogens in the menopause and postmenopause. N Engl J Med 280: 364-367,1967 https://doi.org/10.1056/NEJM196902132800705
  4. Anderson JJB, Garner Sc. Phytoestrogens and bone. Baillieres Best Tract Res Clin Endocrinol Metab 12(4): 543-557, 1998
  5. Setchell KDR, Cassidy A. Dietary isoflavones: biological effects and relevance to human health. J Nutr 129: 758S-767S, 1999
  6. Lee HK. Effect of blackbean and sam-ryung-bak-chul-san on ovariectomy- induced postmenopausal osteoporotic rats. Doctoral Thesis. The Graduated School of Khyung Hee University, 1998
  7. Ryu IC, Lee YM, Koo Y, Bae KW, Chung CP. Effect of safflower extracts on activity of PDL cells and MG-63 cells. J Korean Acad Periodontol 27 (4) : 867-882,1997
  8. Ho JY. Cell proliferation effects of eucommiae cortex and acanthopanacis cortex methanol extracts on the osteoblast-like cell lines, MG-63 and Saos-2. Master thesis. The Graduate School of Kyung Hee University, 2001
  9. Kim MR, Yang CH, Seo BI. Effects of safflower seeds on bone mineral density in ovariectomy-induced postmenopausal osteoporotic rats. Korean J Herbology 13 (2) : 37-43, 1998
  10. Milsicek RJ. Interaction of naturally occurring nonsteroidal estrogens with expressed recombinant human estrogen receptor. J Steroid Biochem Mol Biol 49: 153-160, 1994 https://doi.org/10.1016/0960-0760(94)90005-1
  11. Kim CS, Lee YS, Kim JS, Hahn YH. High performance liquid chromatographic analysis of isoflavones in soybean foods. Korean J Food Sci Technol 32(1): 25-30, 2000
  12. Murkies AL, Wilcox G, Davis SR. Phytoestrogens. J Clin Endocrinol Metab 83 (2): 297-303, 1998 https://doi.org/10.1210/jc.83.2.297
  13. Kang SA, Jang KH, Cho Y, Hong KH, Kong SH, Choue RW. High performance liquid chromatographic analysis of isoflavones in soybean and blackbean. J ARAHE 8 (1) : 44-48, 2001
  14. Kang SA, Jang KH, Cho Y, Hong KH, Suh JH, Choue RW. Effects of artificial stomach fluid and digestive enzymes on the aglycone isoflavone contents of soybean and black bean (Rhynchosia volubilis: Yak-kong). Korean J Nutrition 36 (1): 32-33, 2003
  15. Cho Y, Park S, Shin H, Jang KH, Kang SA, Choue RW. Comparative estrogenic effects of yak-kong and soy bean on the proliferation of human osteoblastic cell line, MG-63. Korean J Nutrition 34(8): 905-911,2001
  16. Kim J, Urn SJ, Woo J, Kim JY, Kim HA, Jang KH, Kang SA, Lim BO, Kang I, Choue RW, Cho Y. Comparative effect of seeds of lhtynchosia volubilis and soybean on MG-63 human osteoblastic cell proliferation and estrogenicity Life Sci, 2005 (In press)
  17. Vidal O, Kindbolom LG, Ohlsson C. Expression and localization of estrogen receptor- $\beta$ in murine and human bone J Bone Miner Res 14(6): 923-929, 1999 https://doi.org/10.1359/jbmr.1999.14.6.923
  18. Lambertini E, Penolazzi L, Aguiari G, del Senno L, Pezzetti F, Sollazzo V, Piva R. Osteoblastic differentiation induced by transcription factor decoy against estrogen receptor a gene. Biochem Biophys Res Commun 292: 761-770,2002 https://doi.org/10.1006/bbrc.2002.6708
  19. Su LF, Knoblauch R, Garabedian MJ. Rho GTPases as modulators of the estrogen receptor transcriptional response. J Biol Chem 276: 3231-3237,2001
  20. Graham FI, Van der AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52: 456-467, 1973 https://doi.org/10.1016/0042-6822(73)90341-3
  21. Kuiper GGJM, Carlsson B, Grandien K, Enmark E. Haggblad J, Nilsson S, Gustafsson JA. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors $\alpha$. and $\beta$. Endocrinology 138: 863-870, 1997 https://doi.org/10.1210/en.138.3.863
  22. Sandy J, Davies M, Prime S, Farndale R. Signal pathways that transduce growth factor-stimulated mitogenesis in bone cells. Bone 23: 17-26, 1998 https://doi.org/10.1016/S8756-3282(98)00067-2
  23. Mohan S, Baylink DJ, Bone growth factors. Clin Orthop Relat Res 263: 30-48, 1991
  24. Kim SW, Lajara R, Rotwein P. Structure and function of a human insulin-like growth factor-I gene promoter. Mol Endocrinol 5: 1964-197, 1991 https://doi.org/10.1210/mend-5-12-1964
  25. Hall LJ, Kajimoto Y, Bichell D, Kim SW, James PL, Counts D, Nixon LJ, Tobin G, Rotwein P. Functional analysis of the rat insulin-like growth factor I gene and identification of an IGF-I gene promoter. DNA Cell Biol 11: 301-313, 1992 https://doi.org/10.1089/dna.1992.11.301
  26. Umayahara Y, Kawamori R, Watada H, !mano E, Iwarna N, Morishima T, Yamasaki Y, Kajimoto Y, Karnada T. Estrogen regulation of the insulin-like growth factor I gene transcription involves an AP-I enhancer. J Biol Chem 269: 16433-16442,1994
  27. Webb P, Nguyen P, Valentine C, Lopez GN, Kwok GR, Mc Inerney E, Katzenellenbogen BS, Enmark E, Gustafsson JA, Nilsson S, Kushner PJ. The estrogen receptor enhances AP-I activity by two distinct mechanisms with different requirements for receptor transactivation functions. Mol Endocrinol 13: 1672-168, 1999 https://doi.org/10.1210/me.13.10.1672