Enhanced Production of Recombinant Protein in Escherichia coli Using Silkworm Hemolymph

  • Kim Ji Eun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim Eun Jeong (School of Chemical and Biological Engineering, Seoul National University) ;
  • Rhee Won Jong (School of Chemical and Biological Engineering, Seoul National University) ;
  • Park Tai Hyun (School of Chemical and Biological Engineering, Seoul National University)
  • Published : 2005.08.01

Abstract

The effect of silkworm hemolymph on the expression of recombinant protein in Escherichia coli was investigated. The addition of silkworm hemolymph to the culture medium in­creased the production of recombinant $\beta$-galactosidase in E. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1, 3, and $5\%$ silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

Keywords

References

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