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Hypoglycemic and Antioxidative Effects of Fermented Chaga Mushroom (Inonotus obliquus) on Streptozotocin-induced Diabetic Rats

Streptozotocin 유발 당뇨쥐에서 발효 차가버섯의 항당뇨 및 항산화 효과

  • Cha Jae-Young (Department of Biotechnology, College of Natural Resources and Life Science,Dong-A University) ;
  • Jun Bang-Sil (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee Chi-Hyeoung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Yooi Ki-Soo (Department of Anatomy, College of Medicine, Dong-A University) ;
  • Moon Jae-Chul (Hong Jae Green Co., Ltd) ;
  • Cho Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 차재영 (동아대학교 응용생명공학부) ;
  • 전방실 (동아대학교 응용생명공학부) ;
  • 이치형 (동아대학교 응용생명공학부) ;
  • 유기수 (동아대학교 의과대학 해부학교실) ;
  • 문재철 (홍재그린(주)) ;
  • 조영수 (동아대학교 응용생명공학부)
  • Published : 2005.10.01

Abstract

The effects of fermented chaga mushroom (Inonotus obliquus) on the concentrations of serum glucose, insulin, lipids and lipid peroxidation in streptozotocin (STZ)-induced diabetic rats were investigated. Rats were fed a semisynthetic diet supplemented with 50 g/kg chaga mushroom powder (the CM group) and fermented chaga mushroom powder (the FCM group), and no supplemented (the control group) for 3 weeks. The polysaccharide concentrations were CM by $42.9\%$ and FCM by $ 39.1\%$, and the total polyphenol concentrations were CM by $ 0.80\%$ and FCM by $0.91\%$. Feed intakes and water consumption, serum glucose, insulin, triglyceride, and blood urea nitrogen concentrations were significantly lower in the FCM group than in both the CM and control groups. The activities of AST and ALT were also significantly lower in the FCM group than in the control group. No significant differences were detected with regard to the serum cholesterol and creatinine concentrations among the experimental groups. Lipid peroxidations in hepatic homogenate, microsomal and mitochondrial subcellular and pancreas were significantly lowered by the administration of FCM in the STZ-diabetic rats. Hepatic glutathione concentrations, which is closely associated with antioxidant system, was significantly higher in the FCM group than in the control group, indicating a marked effect of FCM administration on the endogenous antioxidant system. However, CM treatment showed a moderate antioxidative activity in the STZ-diabetic rats. Our results indicate that fermented chaga mushroom exert hypoglycemic and antioxidative effects in type 1 diabetes mellitus.

차가버섯 (Inonotus obliquus) 분말을 Bacillus sp.로 발효시켜 streptozotocin (STZ)-유발 당뇨쥐에 $ 5\%(w/w)$ 수준으로 첨가한 반합성 식이를 3주간 투여하여 혈당치 및 과산화지질 농도에 미치는 영향을 검토하였다. 차가버섯 및 발효 차가버섯의 다당체는 각각 $42.9\%$$39.1\%$였으며, 폴리페놀 화합물 함량은 각각 $0.80\%$$0.91\%$였다. 식이 및 음료 섭취량, 혈청 중의 glucose, insulin, triglyceride, blood urea nitrogen농도는 당뇨 대조군과 차가버섯 투여군 보다는 발효 차가버섯 투여군에서 현저히 감소하였다. 혈청 중의 간 기능 임상치료 효소인 AST 및 ALT 활성도 발효 차가버섯 투여군에서 역시 감소하였다. 그러나 혈청 total cholesterol 및 creatinine 농도는 각 실험군간에 유의적인 차이는 없었다. 한편, 간 조직의 homogenate, microsomal 및 mitochondria 분획의 과산지질 농도는 당뇨쥐에 발효 차가버섯 투여로 현저히 감소하였다. 간 조직에서 내인성 항산화물질로 알려져 있는 glutathione 농도가 발효 차가버섯 투여군에서 현저히 증가함으로써 과산화지질 농도 억제에 의한 항산화 활성과 밀접한 관련성을 가진 것으로 나타났다 차가버섯 투여군에서는 발효 차가버섯 보다는 미약한 항산화 활성을 보였다. 이상의 결과에서 발효 차가버섯은 streptozotocin-유발 당뇨쥐에서 우수한 항당뇨 및 항산화 효과를 발휘하였다.

Keywords

References

  1. Alarcon-Aguilara, F. L R. Roman-Ramos, S. Perez-Gutierrez, A Aguilar-Contreras, C. C. Contreras-Weber and J. J. Flores-Saenz. 1998. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J. Ethnopharmacol. 61, 101-11 https://doi.org/10.1016/S0378-8741(98)00020-8
  2. AI-Ghaithi, F., M. R. EI-Ridi, E. Adeghate and M. H. Amiri. 2004. Biochemical effects of Citrullus colocynthis in normal and diabetic rats. Mol. Cell. Biochem. 261, 143-149 https://doi.org/10.1023/B:MCBI.0000028749.63101.cc
  3. Awadh-Ali, N. A, R. A A Mothana, A Lesnau, H. Pilgrim and U. Lindequist. 2003. Antiviral activity of Inonotus obliquus hispidus. Fitoterapia. 74, 483-485 https://doi.org/10.1016/S0367-326X(03)00119-9
  4. Baron, H. and P. S. Roheim. 1976. Hyperlipidemia in streptozotocin-treated rats. Diabetes. 25, 509-515 https://doi.org/10.2337/diabetes.25.6.509
  5. Benoit, M. A., G. D. Aprano and M. Lacroix. 2000. Effect of ${\gamma}$-irradiation on phenylalanine ammonia-lyase activity, total phenolic content, and respiration of mushrooms (Agaricus bisporus). J. Agric. Food. Chem. 48, 6312-6316 https://doi.org/10.1021/jf000543s
  6. Berger, A, D. Rein, E. Kratky, J. Monnard, H. Hajjaj, J. Meirim, C. Piguet-Welsch, J. Hau, K. Mace and P. Niederberger. 2004. Cholesterol-lowering properties of Ganoderma lucidum in vitro, ex vivo, an in hamsters and minipigs. Lipid. Health. Disease. 3-12
  7. Beutler, E., O. Duron and B. M. Kelly. 1963. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61, 882-88
  8. Cha, J. Y., B. S. Jeon, J. W. Park, J. C. Moon and Y. S. Cho. 2004. Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells. J. Korean. Soc. Appl. Biol. Chem. 47, 202-207
  9. Cha, J. Y., B. S. Jeon, J. W. Park, G. G. Shin, B. K. Kim, D. W. Bae, J. H. Yoo, B. S. Jeon and Y. S. Cho. 2004. Antioxidative effects of mushroom extract and fermented milk containing its extract on in vivo and in vitro lipid peroxidation. J. Life. Sci. 14, 514-520 https://doi.org/10.5352/JLS.2004.14.3.514
  10. Cha, J. Y., Y. Mameda, K. Oogami, K. Yamamoto and T. Yanagita. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats. Biosci. Biotech. Biochem. 62, 508-513 https://doi.org/10.1271/bbb.62.508
  11. Cha, J. Y., B. S. Jeon, J. W. Park, G. G. Shin, B. K. Kim, H. K. Kim and Y. S. Cho. 2004. Hypoglycemic effect of mushroom fermented milk in streptozotocin induced diabetic rats. J. Life. Sci. 14, 676-682 https://doi.org/10.5352/JLS.2004.14.4.676
  12. Chang, S. T. 1999. Wrold production of cultivated edible and medicinal mushrooms in 1977 with emphasis on Lintinus edodes (Berk) Sig. in China. Int. J. Med. Mushrooms. 1, 291-300 https://doi.org/10.1615/IntJMedMushr.v1.i4.10
  13. Coseteng, M. Y. and C. Y. Lee. 1987. Changes in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning. J. Food. Sci. 52, 985-989 https://doi.org/10.1111/j.1365-2621.1987.tb14257.x
  14. Duncan, D. B. 1957. Multiple range test for correlated and heteroscedastic means. Biometrics. 13, 164-176 https://doi.org/10.2307/2527799
  15. Fukushima, M., T. Ohashi, Y. Fujiwara, K. Sonoyama and M. Nakano. 2001. Cholesterol-lowering effects of maitake (Grofola frondosa) fiber, shiitake (Letinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp. Biol. Med. 226, 758-765 https://doi.org/10.1177/153537020222600808
  16. Gray, A. M. and P. R. Flatt. 1998. Insulin-releashing and insulin-like activity of Agaricus campestris (mushroom). J. Endocrinol. 157, 259-266 https://doi.org/10.1677/joe.0.1570259
  17. Ham, S. S., S. W. Oh, Y. K. Kim, K. S. Shin, K. Y. Chang and G. H. Chung. 2003. Antioxidant and genotoxic inhibition activity of ethanol extract from the Inonotus obliquus. J. Korean. Soc. Food. Sci. Nutr. 32, 1071-1075 https://doi.org/10.3746/jkfn.2003.32.7.1071
  18. Jeon, B.S., J. W. Park, B. K. Kim, H. K. Kim, T. S. Jung, J. R. Hahm, D. R. Kim, Y. S. Cho and J. Y. Chao 2005. Fermented mushroom milk supplemented dietary fiber prevents the onset of obesity and hypertriglyceridemia in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Diabetes. Obesity. Met. 7, 709-715 https://doi.org/10.1111/j.1463-1326.2005.00456.x
  19. Jeon, B. S., J. W. Park, G. G. Shin, B. K. Kim, H. K. Kim, Y. S. Cho, J. Y. Chao 2004. Effect of fermented mushroom milk on hyperlipidemia and hepatic injury in streptozotocin-induced diabetic and Zucker diabetic fatty rats. Food. Sci. Biotechnol. 13, 576-580
  20. Jia, D. M., A. Tabaru, T. Akiyama, S. Ade and M. Otsuki. 2000. Fatty liver, chronic viral hepatities and autoimmune hepatities. Troglitazone prevents fatty changes of the liver in obese diabetic rats. J. Gastroentrol. Hepatol. 15, 1183-1191 https://doi.org/10.1046/j.1440-1746.2000.02316.x
  21. Kim, M. Y., M. H. Park and G. H. Kim. 1997. Effects of mushroom protein-bound polysaccharides on the blood glucose levels and energy metabolism in streptozotocin-induced diabetic rats. J. Kor. Nutr. 30, 743-750
  22. Kim, B. K., G. G. Shin, B. S. Jeong and J. Y. Chao 2001. Cholesterol-lowering effect of mushrooms powder in hyperlipidemic rats. J. Korean. Soc. Food. Sci. Nutr. 30, 510-515
  23. Konno, S., D. G. Tortorelis, S. A. Fullerton, A. A. Samadi, J. Hettiarachchi and H. Tazaki. 2001. A possible hypoglycaemic effect of Maitake mushroom on type 2 diabetic patients. Diabetic. Med. 18, 1010-1011 https://doi.org/10.1046/j.1464-5491.2001.00532-5.x
  24. Kubok, K., H. Aoki and N. Naba, 1994. Anti-diabetic activities present in the fruiting body of Gnfola frondosa (mitake). Biol. Pharm. Bull. 17, 1106-1110 https://doi.org/10.1248/bpb.17.1106
  25. Lee, E. J. and H. D. Jang. 2004. Antioxidant activity and protective effect of five edible mushrooms on oxidative DNA damage. Food. Sci. Bioteehnol. 13, 443-449
  26. Lee, H. W., T. S. Park, J. W. Choi, S. Y. Yi and W. S. Shin. 2003. Antidiabetic effects of chitosan oligosaccharides in neonatal streptozotocin-induced noninsulin-dependent diabetes mellitus in rats. Biol. Pharm. Bull. 26, 1100-1103 https://doi.org/10.1248/bpb.26.1100
  27. Lee, J. J., H. Y. Yi, J. W. Yang, J. S. Shin, J. H. Kwon and C. W. Kim. 2003. Characterization of streptozotocin-induced diabetic rats and pharmacodynamic activity of insulin formation. Biosci. Bioteehnol. Bioehem. 67, 2396-2401 https://doi.org/10.1271/bbb.67.2396
  28. Lee, E. W., P. He, H. Kawagishi and K. Sugiyama. 2000. Suppression of D-galactosamine-induced liver injury by mushroom in rats. Biosei. Bioteehnol. Bioehem. 64, 2001-2002
  29. Lee, S. H., Y. M. Han, B. H. Min and J. S. Park. 2003. Cytoprotective effects of polyenoylphosphatidylcholine (PPC) on beta-cells during diabetic induction by streptozotocin. J. Histoehem. Cytoehem. 51, 1005-1015 https://doi.org/10.1177/002215540305100804
  30. Lu, X. J., X. M. Chen, D. X. Fu, W. Cong and F. Ouyang. 2002. Effect of Amorphophallus konjae oligosaccharides on STZ-induced diabetic model of isolated islets. Life. Sci. 72, 711-719 https://doi.org/10.1016/S0024-3205(02)02303-2
  31. Maritim, A C., R. A Sanders and J. BWatkins. 2003. 3rd. Diabetes, oxidative stress, and antioxidants: a review. J. Bioehem. Mol. Toxieol. 17, 24-38 https://doi.org/10.1002/jbt.10058
  32. Mau, J. L., H. C. Un and S. F. Song. 2002. Antioxidant properties of several specially mushrooms. Food. Res. Inter. 35, 519-526 https://doi.org/10.1016/S0963-9969(01)00150-8
  33. Mizuno, T., C. Zhuang, K. Abe, H. Okamoto, T. Kiho, S. Ukai, S. Leclerc and J. Meijer. 1999. Antitumor and hypoglycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fe.) Pll. (Aphyllophoromycetideae). Int. J. Med. Mushrooms. 1, 301-316 https://doi.org/10.1615/IntJMedMushr.v1.i4.20
  34. Mizuno, M., K. Morimoto and H. Tsuchida. 1993. Polysaccharides from Agurieus blazei stimulate lymphocyte T-cell subsets in mice. Biosei. Bioteehnol. Bioehem. 62, 434-437
  35. Miyake, Y., K. Yamamoto, N. Tsujihara and T. Osawa. 1998. Protective effects of lemon flavonoids on oxidative stree in diabetic rats. Lipids. 33, 689-695 https://doi.org/10.1007/s11745-998-0258-y
  36. Nicotera, P. and S. Orrenius. 1986. Role of thiols in protection against biological reactive intermediates. Adv. Exp. Med. Biol. 197, 41-49 https://doi.org/10.1007/978-1-4684-5134-4_4
  37. Ohaeri, O. C. 2001. Effect of garlic oil on the levels of various enzymes in the serum and tissue of streptozotocin diabetic rats. Bioscience. Reports. 21, 19-24 https://doi.org/10.1023/A:1010425932561
  38. Prince, P. S. M. and V. P. Menon. 2001. Antioxidant action of Tinospora eordifolia root extract in alloxan diabetic rats. Phytother. Res. 15, 213-218 https://doi.org/10.1002/ptr.707
  39. Sanders, R. A, F. M. Rauscher and J. B. Watkins. 2001. 3rd. Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J. Bioehem. Mol. Toxieol. 15, 143-149 https://doi.org/10.1002/jbt.11
  40. Talpur, N. A, B. W. Echard, A Y. Fan, O. Jaffari, D. Bagchi and H. G. Preuss. 2002. Antihypertensive and metabolic effects of whole Maitake mushroom powder and its fractions in two rat strains. Mol. Cell Bioehem. 237, 129-136 https://doi.org/10.1023/A:1016503804742
  41. Traverso, N., S. Menini, P. Odetti, M. A Pranzato, D. Cattalosso and U. M. Marinari. 1999. Lipidoperoxidation in hepatic subcellilar compartments of diabetic rats. Free. Radic. Bio. Med. 26, 538-547 https://doi.org/10.1016/S0891-5849(98)00238-X
  42. Uchiyama, M. and M. Mihara. 1978. Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86, 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  43. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbial. Biotechnol. 60, 258-274 https://doi.org/10.1007/s00253-002-1076-7
  44. Yamamoto, H., Y. Uchigata and H. Okamoto. 1981. DNA strand breaks in pancreatic islets by in vivo administration of alloxan or streptozotoxin. Biochem. Biopys. Res. Commun. 103, 1014-1020 https://doi.org/10.1016/0006-291X(81)90910-4
  45. Yang, B. K., D. H. Kim, S. C. Jeong, S. Das, Y. S. Choi, J. S. Shin, S. C. Lee and C. H. Song. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotechnol. Biochem. 66, 937-942 https://doi.org/10.1271/bbb.66.937
  46. Yuan, Z., P. He, J. Cui and H. Takeuchi. 1998. Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricula-judae Quel. on genetically diabetic KK-Ay mice. Biosci. Biotechnol. Biochem. 62, 1898-1903 https://doi.org/10.1271/bbb.62.1898
  47. Zhang, G. L., Y. H. Wang, W. Ni, H. L. Teng and Z. B. Un. 2002. Hepatoprotective role of Ganodemza lucidum polysaccharide against BCG-induced immune liver injury in mice. World. J. Gastroenterol. 8, 728-733 https://doi.org/10.3748/wjg.v8.i4.728
  48. Zhang, H. N., J. H. He, L. Yuan and Z. B. Lin. 2003. In vitro and in vivo protective effects of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life. Sciences. 73, 2307-2319 https://doi.org/10.1016/S0024-3205(03)00594-0
  49. Zhang, H. N. and Z. B. Un. 2004. Hypoglycemic effect of Ganoderma lucidum polysaccharides. Acta. Pharmacal. Sin. 25, 191-195

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