Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improvement of Photoheterotrophic Hydrogen Production of Rhodobacter sphaeroides by Removal of B800-850 Light-Harvesting Complex

  • KIM EUI-JIN (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • YOO SANG-BAE (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • KIM MI-SUN (Biomass Research Team, Korea Institute of Energy Research) ;
  • LEE JEONG K. (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
  • Published : 2005.10.01
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Abstract

The photoheterotrophic $H_2$ production of Rhodobacter sphaeroides was significantly increased through disruption of the genes coding for uptake hydrogenase and poly-${\beta}$-hydroxybutyrate (PHB) synthase (Lee et al., Appl. Microbiol. Biotechnol. 60: 147-153, 2002). In this work, we further removed the B800-850 light-harvesting (LH) complex from the strain and found an increase in $H_2$ production at the light-saturating cell growth (${\ge}10$ Watts $[W]/m^2$). Neither the mutant nor the wild-type produced more $H_2$ at the brighter light. Accordingly, light does not appear to be limited for the $H_2$ production by the presence of B800-850. However, increase in the level of the spectral complexes resulted in decrease of $H_2$ production. Thus, although the B875 is essential for light harvesting, the consumption of cellular energy for the synthesis of B800-850 and the surplus LH complexes may reduce the energy flow into the $H_2$ production of R. sphaeroides.

Keywords

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