Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Statistical Approach to Development of Culture Medium for Ansamitocin P-3 Production with Actinosynnema pretiosum ATCC 31565

  • BANDI SRINIVASULU (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • KIM YOON JUNG (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • SA SOON OK (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • CHANG YONG-KEUN (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 2005.10.01
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Abstract

The Plackett-Burman design and the response surface method (RSM) with a central composite design (CCD) were employed to develop a culture medium for ansamitocin P-3 production using Actinosynnema pretiosum ATCC 31565. Among the 11 nutrients tested using the Plackett-Burman design, two carbon sources, sucrose and dextrin, and two nitrogen sources, polypeptone and yeast extract, were selected. Optimization of the concentrations of the selected nutrients was then performed using RSM with CCD. After two rounds of RSM, the optimum concentrations ($\%w/v$) of sucrose, dextrin, polypeptone, and yeast extract were identified as 4.5, 4.5, 0.16, and 0.89, respectively. The maximum ansamitocin P-3 titer was 45.2 mg/l with the optimized medium, which was about 6 times higher than that (7.315 mg/l) obtained with an $R_{2}YE$ medium before optimization.

Keywords

References

  1. Abdel-Fattah, Y. R. and Z. A. Olama. 2002. L-Asparaginase production by Pseudomonas aeruginosa in solid-state culture: Evaluation and optimization of culture conditions using factorial designs. Process Biochem. 38: 115-122 https://doi.org/10.1016/S0032-9592(02)00067-5
  2. Aknazarov, S. and V. Kafarov. 1982. Experiment Optimization in Chemistry and Chemical Engineering. Mir Publications, Moscow, Russia
  3. Asai, M., E. Mizuta, M. Izawa, K. Haibar, and T. Kishi. 1978. Isolation, chemical characterization and structure of ansamitocin, a new antitumor ansamitocin antibiotic. Tetrahedron 35: 1079-1085 https://doi.org/10.1016/S0040-4020(01)93726-3
  4. Baek, K., H. J. Shin, H. H. Lee, Y. S. Jun, and J. W. Yang. 2002. Statistical modeling of electrochemical removal of sodium in fermented food composts. Korean J. Chem. Eng. 19(4): 627 -631 https://doi.org/10.1007/BF02699308
  5. Bloor, S. and R. R. England. 1981. Elucidation and optimization of the medium constituents controlling antibiotic production by the Cyanobacterium Nostoc muscorum. Enz. Microb. Technol. 13: 76-81
  6. Box, G. E. P. and K. B. Wilson. 1951. On the experimental attainment of optimum conditions. J. Roy. Stat. Soc. B13: 1-45
  7. Box, G. E. P., W. G. Hunter, and J. S. Hunter. 1978. Statistics for Experiments, pp. 291-334. John Wiley and Sons, NY, U.S.A
  8. Chang, Y. N., J. C. Huang, C. C. Lee, I. L. Shih, and Y. M. Tzeng. 2002. Use of response surface methodology to optimize culture medium for production of lovastatin by Monascus ruber. Enz. Microb. Technol. 30: 889-894 https://doi.org/10.1016/S0141-0229(02)00037-6
  9. Chen, Q. H., G. Q. He, and M. A. M. Ali. 2002. Optimization of medium composition for the production of elastage by Bacillus sp. EL 31410 with response surface methodology. Enz. Microb. Technol. 30: 667-672 https://doi.org/10.1016/S0141-0229(02)00028-5
  10. Cochran, W. G. and G. M. Cox. 1957. Experimental Designs, pp. 346-354. 2nd Ed. John Wiley and Sons, NY, U.S.A
  11. Devore, J. L. 1995. Probability and Statistics for Engineering and the Sciences, p. 710. 4th Ed. Duxbury Press, An International Thomson Publishing Company, US
  12. Dulaney, E. L. 1948. Observation on Streptomyces griseous II. Nitrogen sources for growth and streptomycin production. J. Biotechnol. 56: 305
  13. Fulston, M., A. L. Stefanska, and J. E. Thirkettle. 2003. Methods for Ansamitocin Production. US Patent 6,573,074
  14. Hasegawa, T., S. Tanida, K. Hatano, E. Highashide, and M. Yoneda. 1983. Motile actinomycetes: Actinosynnema pretiosum subsp. pretiosum sp. Nov. subsp. nov., and Actinosynnema pretiosum subsp. auranticum subsp. novo. Int. J. Syst. Bacteriol. 33: 314-320 https://doi.org/10.1099/00207713-33-2-314
  15. Hatano, K., M. Nakamishi, and S. I. Akiyama. Method for the Production of Antibiotic C-15003 P-3. US Patent 4,356,265
  16. Higashide, E., M. Asai, K. Ootsu, S. Tanida, Y. Kozai, T. Hasegawa, T. Kishi, Y. Sugino, and M. Yoneda. 1977. Ansamitocin, a group of novel maytansinoid antibiotic with antitumor properties from Nocardia. Nature (London) 270: 721-722 https://doi.org/10.1038/270721a0
  17. Hopwood, D. A. 1986. Genetic mapping by conjugation in Streptomyces spp. Chapter 15, pp. 191-197. In Demain, A. L. and Solomon, N. A. (eds.). Manual of Industrial Microbial and Biotechnology
  18. Houng, J. Y., H. F. Hsu, Y. H. Liu, and J. Y. Wu. 2003. Applying the Taguchi robust design to the optimization of the asymmetric reduction of ethyl 4-chloro acetoacetate by Bakers' yeast. J. Biotechnol. 100: 239-250 https://doi.org/10.1016/S0168-1656(02)00179-7
  19. Jeong, D. H., K. D. Park, S. H. Kim, K. R. Kim, S. W. Choi, J. T Kim, K. H. Choi, and J. H. Kim. 2004. Identification of Streptomyces sp. producing antibiotics against phytopathogenic fungi, and its structure. J. Microbiol. Biotechnol. 14: 212-215
  20. Khuri, A. I. and J. A. Cornell. 1987. Response Surface Designs. Marcel Dekker Inc. NY, U.S.A
  21. King, V. A. 1993. Studies on the control of the growth of Saccharomyces cerevisiae by using response surface methodology to achieve effective preservation at high water activities. Int. J. Food Sci. Technol. 28: 519-529
  22. Kupchan, S. M, Y. Komada, W. A. Court, G. J. Thomas, R. M. Smith, A. Karim, C. J. Gilmore, R. C. Haltiwanger, and R. F. Bryan. 1972. Maytansine, a novel antileukemic ansa macrolide from May tenus ovatus. J. Amer. Chem. Soc. 94: 1354-1356 https://doi.org/10.1021/ja00759a054
  23. Lai, L. S. T, C. C. Pan, and B. K. Tzeng. 2003. The influence of medium design on lovastatin production and pellet formation with a high-producing mutant of Aspergillus terreus in submerged cultures. Process Biochem. 38: 1317-1326 https://doi.org/10.1016/S0032-9592(02)00330-8
  24. Lidino, C. A., R. V. J. Chari, L. A. Bourret, N. L. Kedersha, and V. S. Goldmacher. 1997. Folate-maytansinoids: Target selective drugs of low molecular weight. Int. J. Cancer 73: 859-864 https://doi.org/10.1002/(SICI)1097-0215(19971210)73:6<859::AID-IJC16>3.0.CO;2-#
  25. Liu, C., B. M. Tadayoni, L. A. Bourret, K. M. Mattocks, S. M. Derr, W. C. Widdison, N. L. Kedersha, P. D. Ariniello, V. S. Goldmacher, and J. M. Lambert. 1996. Eradiation of large colon tumor xenografts by targeted delivery of Maytansinoids. Proc. Natl. Acad. Sci. USA 93: 8618-8623
  26. Ma, A. Y. M. and B. Ooraikul. 1986. Optimization of enzymatic hydrolysis of canola meal with response surface methodology. J. Food Proc. Preserv. 10: 99-113 https://doi.org/10.1111/j.1745-4549.1986.tb00010.x
  27. Kim, M. W., C. S. Shin, H. J. Yang, S. H. Kim, H. Y. Lim, e. H. Lee, M. K. Kim, and Y. H. Lim 2004. Naltriben analogues as peptide anticancer drugs. J. Microbiol. Biotechnol. 14: 881-884
  28. Murthy, M. V. R., E. V. S. Mohan, and A. K. Sadhukhan. 1999. Cyclosporin-A production by Tolypocladium inflatum using solid state fermentation. Process Biochem. 34: 269-280 https://doi.org/10.1016/S0032-9592(98)00095-8
  29. Park, J. S., B. H. Kim, J. H. Lee, E. S. Seo, K. S. Cho, H. J. Park, H. K. Kang, S. K. Yoo, M. S. Ha, H. J. Chung, D. L. Cho, D. F. Day, and D. Kim. 2003. Optimization of novel glucanhydrolase production of Lipomyces starkeyi KSM 22 by statistical design. J. Microbiol. Biotechnol. 13: 993-997
  30. Plackett, R. L. and J. P. Burman. 1946. The design of optimum multifactorial experiments. Biometrika 33: 305-325 https://doi.org/10.1093/biomet/33.4.305
  31. Rao, K. J., C. H. Kim, and S. K. Rhee. 2000. Statistical optimization of medium for the production of recombinant hirudin from Saccharomyces cerevisiae using response surface methodology. Process Biochem. 35: 639-647 https://doi.org/10.1016/S0032-9592(99)00129-6
  32. Rosi, I., L. Costamagna, and M. Bertuccioli. 1987. Wine fermentation by immobilized yeast: An optimization study, In: Flavor Science and Technology. John Wiley and Sons
  33. Sathosh, A., H. Ogawa, and Y. Satomura. 1976. Regulation of N-acetyl kanamycin amidohydrolase in the idophase in kanamycin fermentation. Agric. Biol. Chem. 40: 191
  34. Tanida, S., T. Hasegawa, K. Hatano, E. Highashide, and M. Yoneda. 1980. Ansamitocins, maytansinoids. Antitumor antibiotics producing organism, fermentation and antimicrobial activities. J. Antibiotics (Tokyo) 32: 192-198
  35. Theodore, K. and T. Panda. 1995. Application of response surface methodology to evaluate the influence of temperature and initial pH on the production of ${\beta}$-1,3-glucanase and carboxy methyl cellulose from Trichoderma harzianum. Enz. Microb. Technol. 17: 1043-1049 https://doi.org/10.1016/0141-0229(94)00029-8
  36. Yu, T.-W., L. Bai, D. Clade, D. Hoffinann, S. Toelzer, K. Q. Trinh, J. Xu, S. J. Moss, E. Leistner, and H. G. Floss. 2002. The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. Proc. Natl. Acad. Sci. USA 99: 7968- 7973