Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Diversity of Denitrifying Bacteria Isolated from Daejeon Sewage Treatment Plant

  • Lim Young-Woon (Department of Wood Science, University of British Columbia) ;
  • Lee Soon-Ae (Department of Biotechnology, Hannam University) ;
  • Kim Seung Bum (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Yong Hae-Young (Department of Biotechnology, Hannam University) ;
  • Yeon Seon-Hee (Department of Biotechnology, Hannam University) ;
  • Park Yong-Keun (Laboratory of Microbial Genetics, School of Life Science and Biotechnology, Korea University) ;
  • Jeong Dong-Woo (Department of Biotechnology, Hannam University) ;
  • Park Jin-Sook (Department of Biotechnology, Hannam University)
  • Published : 2005.10.01
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Abstract

The diversity of the denitrifying bacterial populations in Daejeon Sewage Treatment Plant was examined using a culture-dependent approach. Of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. According to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. This grouping was supported by an unweighted pair group method, using an arithmetic mean (UPGMA) analysis with fatty acid profiles. Restriction pattern analysis of 16S rDNA with four endonucleases (AluI, BstUI, MspI and RsaI) again revealed seven distinct groups, consistent with those defined from the morphological and biochemical properties and fatty acid profiles. Through the phylogenetic analysis using the 16S rDNA partial sequences, the main denitrifying microbial populations were found to be members of the phylum, Proteobacteria; in particular, classes Gammaproteobacteria (Aeromonas, Klebsiella and Enterobacter) and Betaproteobacteria (Acidovorax, Burkholderia and Comamonas), with Firmicutes, represented by Bacillus, also comprised a major group.

Keywords

References

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