The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Development of Rapid Analysis Method for Pesticide Residues by GC-MS/MS

GC-MS/MS를 이용한 잔류농약 신속검사법 개발

  • Choi, Yong-Hoon (Testing and Analysis Team, Gyeongin Regional Office, Korea Food and Drug Administration) ;
  • Nam, Hye-Seon (Testing and Analysis Team, Gyeongin Regional Office, Korea Food and Drug Administration) ;
  • Hong, Hye-Mi (Department of Food and Nutrition, Inha University) ;
  • Lee, Jin-Ha (Testing and Analysis Team, Gyeongin Regional Office, Korea Food and Drug Administration) ;
  • Chae, Kab-Ryong (Testing and Analysis Team, Busan Regional Office, Korea Food and Drug Administration) ;
  • Lee, Jong-Ok (Food Contaminants Team, Korea Food and Drug Administration) ;
  • Kim, Hee-Yun (Testing and Analysis Team, Gyeongin Regional Office, Korea Food and Drug Administration) ;
  • Yoon, Sang-Hyeon (Testing and Analysis Team, Gyeongin Regional Office, Korea Food and Drug Administration)
  • 최용훈 (경인지방식품의약품안전청 시험분석팀) ;
  • 남혜선 (경인지방식품의약품안전청 시험분석팀) ;
  • 홍혜미 (인하대학교 식품영양학과) ;
  • 이진하 (경인지방식품의약품안전청 시험분석팀) ;
  • 채갑용 (부산지방식품의약품안전청 시험분석팀) ;
  • 이종옥 (식품의약품안전청 식품오염물질팀) ;
  • 김희연 (경인지방식품의약품안전청 시험분석팀) ;
  • 윤상현 (경인지방식품의약품안전청 시험분석팀)
  • Published : 2005.12.30
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Abstract

Condition of Ion-Trap gas chromatography-mass spectrometry (GC-MS) for rapid screening of 206-pesticides residues in agricultural foodstuffs was optimized. As applying a wide-bore column (10 m${\times}$0.53 mm, DF 0.25 um) connected with a fused silica restrictor (0.6 m${\times}$0.1 mm), a significant retention time reduction was obtained. Additionally, the shape of peaks was sharper and higher than classical GC's and GC-MS's, which allowed lower detection limits. To easily manage many spectral data, both of Electron Ionization(EI) and Chemical Ionization(CI) techniques were adopted in screening procedure. At the following steps, MS-MS technique were used to confirm screened analytes in complicated matrices.

206종의 농약성분을 신속하게 분석하기 위한 가스크로마토그래피-질량분석기 이용방법을 최적화 하였다. Wide-bore 컬럼을 채택함으로써 기존의 캐필러리 컬럼을 이용할 때에 비해 분리능을 비슷하게 유지하면서도 분석시간이 1/2이상 단축되고, 감도는 더욱 개선되었다. 시료의 성분이 복잡하고 분석대상 농약이 많은 경우에도, EI와 CI 스크리닝 결과를 비교함으로써 방해물질에 의한 허위정보(False Positive)를 상쇄할 수 있었다. 허위정보에 대한 위험이 적어지면서 시험용액의 정제를 간소화 할 수 있어 전체적인 분석과정을 간소화 할 수 있었다. 또한 검출이 예상되는 성분에 대해서만 확인실험을 함으로써 검사시간을 절약하고 검사의 정확도를 향상시킬 수 있었다.

Keywords

References

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