Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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PROBLEMS IN OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

쥐의 골수로부터 추출한 줄기세포를 이용한 조골세포로의 분화 유도과정에서 나타난 문제점에 관한 분석 연구

  • Kim, In-Sook (Dental Research Institute, Seoul National University) ;
  • Cho, Tae-Hyung (Dental Research Institute, Seoul National University) ;
  • Zhang, Yu-Lian (Dental Research Institute, Seoul National University) ;
  • Lee, Kyu-Back (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University) ;
  • Park, Yong-Doo (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University) ;
  • Rho, In-Sub (Chemical Engineering Department, Seoul National University of Technology) ;
  • Weber, F. (Lab. for Osteology, Dept. of Craniomaxillofacial Surgery, University Hospital Zurich) ;
  • Lee, Jong-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Myung-Jin (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Hwang, Soon-Jung (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 김인숙 (서울대학교 치학연구소) ;
  • 조태형 (서울대학교 치학연구소) ;
  • 장옥련 (서울대학교 치학연구소) ;
  • 이규백 (고려대학교 의과대학 의공학교실, BK21 의과학사업단) ;
  • 박용두 (고려대학교 의과대학 의공학교실, BK21 의과학사업단) ;
  • 노인섭 (서울산업대학교 화학공학과) ;
  • ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김명진 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 황순정 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.01.31
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Abstract

This study was aimed to characterize osteogenic potential of rat bone marrow stromal cells (BMSC) isolated with standard flushing method and investigate the plasticity of transdifferentiation between osteoblastic and adipocytic lineage of cultured BMSC. Unlike aspiration method in human, rat bone marrow was extracted by means of irrigation with culture media that elevates the possibility of co-extraction of committed osteoprogenitor, or preosteoblast or other progenitor cells of several types present inside bone marrow. The cultured stromal cells showed high ALP activity which is representative marker of osteoblast without any treatment. Osteogenic inducers such as Dex and BMP-2 were examined for the evaluation of their effect on osteogenic and adipocytic differentiation of stromal cells, because they function as osteoinductive agent in stromal cells, but simultaneously induce adipogenic differentiation. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity or mRNA expression of osteoblast markers such as osteopontin, bone sialoprotein, collagen type I and CbfaI, and in vitro matrix mineralization by von Kossa staining. Oil red staining method was used to detect adipocyte and adipocytic marker, aP2 and $PPAR{\gamma}2$ expression was examined using RT-PCR. It can be supposed that irrigation procedure resulted in high portion of already differentiation-committed osteoprogenitor cell showing elevated ALP activity and strong mineralization only under the supplement of $100{\mu}M$ ascorbic 2-phosphate and 10mM ${\beta}$-glycerophosphate without any treatment of osteogenic inducers such as Dex and BMP-2. Dex and BMP-2 seemed to transdifferentiate osteoprogenitor cells having high ALP activity into adipocytes temporarily, but continuous treatment redifferentiated into osteoblast and developed in vitro matrix mineralization. This property must be considered either in tissue engineering for bone regeneration, or in research of characterization of osteogenic differentiation, with rat BMSC isolated by the standard irrigation method.

Keywords

References

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