Mycobiology

The Korean Society of Mycology (한국균학회)
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Effect of Iron Availability on Induction of Systemic Resistance to Fusarium Wilt of Chickpea by Pseudomonas spp.

  • Saikia, Ratul (National Bureau of Agriculturally Important Microorganisms (NBAIM)) ;
  • Srivastava, Alok K. (Laboratory of Microbial Biotechnology, P G Department of Botany, SMMT P G College) ;
  • Singh, Kiran (Department of Microbiology, Barkatullah University) ;
  • Arora, Dilip K. (National Bureau of Agriculturally Important Microorganisms (NBAIM)) ;
  • Lee, Min-Woong (Department of Agrobiology, Dongguk University)
  • Published : 2005.03.30
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Abstract

Selected isolates of Pseudomonas fluorescens (Pf4-92 and PfRsC5) and P. aeruginosa (PaRsG18 and PaRsG27) were examined for growth promotion and induced systemic resistance against Fusarium wilt of chickpea. Significant increase in plant height was observed in Pseudomonas treated plants. However, plant growth was inhibited when isolates of Pseudomonas were used in combination with Fusarium oxysporum f. sp. ciceri (FocRs1). It was also observed that the Pseudomonas spp. was colonized in root of chickpea and significantly suppressed the disease in greenhouse condition. Rock wool bioassay technique was used to study the effect of iron availability on the induction of systemic resistance to Fusarium wilt of chickpea mediated by the Pseudomonas spp. All the isolates of Pseudomonas spp. showed greater disease control in the induced systemic resistance (ISR) bioassay when iron availability in the nutrient solution was low. High performance liquid chromatography (HPLC) analysis indicated that an the bacterial isolates produced more salicylic acid (SA) at low iron ($10\;{\mu}M$ EDDHA) than high iron availability ($10\;{\mu}Fe^{3+}$ EDDHA). Except PaRsG27, all the three isolates produced more pseudobactin at low iron than high iron availability.

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References

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