Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Developing a Protein-chip for Depigmenting Agents Screening

미백제 스크리닝용 단백질칩의 개발

  • Kim, Eun-Ki (Department of Biological Engineering, Inha University) ;
  • Kwak, Eun-Young (Department of Biological Engineering, Inha University) ;
  • Han, Jung-Sun (Department of Biological Engineering, Inha University) ;
  • Lee, Hyang-Bok (Department of Biological Engineering, Inha University) ;
  • Shin, Jung-Hyun (Department of Dermatology, College of Medicine, Inha University)
  • 김은기 (인하대학교 공과대학 생명화학공학부 생물공학과) ;
  • 곽은영 (인하대학교 공과대학 생명화학공학부 생물공학과) ;
  • 한정선 (인하대학교 공과대학 생명화학공학부 생물공학과) ;
  • 이향복 (인하대학교 공과대학 생명화학공학부 생물공학과) ;
  • 신정현 (인하대학교 의과대학 피부과)
  • Published : 2005.03.01
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Abstract

For the high-throughput-screening system (HTS) of depigmenting agents using a protein chip, effects of oligonucleotide-inhibitor sequence on the binding of Mitf protein to E box of MC1R was investigated. The sequence of oligonucletide-inhibitor affected the binding of the target DNA to Mitf, depending on the location of the sequence variation in the inhibitor nucleotide. The oligonucletide-inhibitor that changed the CATGTG sequence didn't show enough inhibition of the target DNA to Mitf, whereas significant inhibition was observed when the sequence outside the CATGTG was changed. This result indicated that CATCTG is crucial sequence for the binding of Mitf to I-box which initiates the transcription of pigmenting genes.

미백 물질 탐색 방법으로, MC1R 발현 인자인 Mitf (microrhthalmia transcription factor)를 이용한 protein chip을 적용하였다. MC1R promoter와 Mitf 결합의 저해 인자로써, DNA 상의 결합 부위인 E-box (CATGTG)와 유사한 서열을 가진 oligomer를 사용하였고, E-box 내외부의 서열 변화에 따른 저해율 또한 측정하였다. 그 결과 DNA-Mitf 결합 저해율에 있어서, E-box 서열 내 변화를 준 oligonucleotide 경쟁자는, E-box 이외의 서열 변화를 준 경쟁자보다 낮은 수치를 보였다.

Keywords

References

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