The Concentration of Tartrate Resistant Acid Phosphatase in Synovial Fluid of Canine Stifle Joint

정상 개의 관절액에서 TRAP(Tartrate resistant acid phosphatase)농도 측정

  • Lee Hae-beom (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Alam Md.Rafiqul (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Choi Sung-jin (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Park Sang-youel (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Lee Young-hoon (School of Dentistry, Chonbuk National University) ;
  • Chon Seung-ki (Chon Seung Ki Animal Clinic) ;
  • Choi In-hyuk (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim Nam-soo (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University)
  • 이해범 (전북대학교 수의학과대학) ;
  • 알람 (전북대학교 수의학과대학) ;
  • 최성진 (전북대학교 수의학과대학) ;
  • 박상렬 (전북대학교 수의학과대학) ;
  • 이영훈 (전북대학교 치과대학) ;
  • 전승기 (전승기 동물병원) ;
  • 최익혁 (전북대학교 수의학과대학) ;
  • 김남수 (전북대학교 수의학과대학)
  • Published : 2005.09.01

Abstract

The concentration of tartrate resistant acid phosphatase (TRAP) in synovial fluid of normal stifle joint was investigated in order to establish a baseline data to distinguish between healthy joint and joint with injury of cranial cruciate ligament (CCL). Twenty three mixed-breed healthy dogs free from joint diseases (fourteen adult and nine young) were used in this study. The dogs were sedated and synovial fluid was collected from the femoropatellar compartment of stifle joints by direct arthrocentesis. The concentration of TRAP in synovial fluid was determined using the method of Lang. The concentration of TRAP were $0.083{\pm}0.039$ IU/ml in adult dogs, $0.064{\pm}0.023$ IU/ml in young dogs, $0.075{\pm}0.028$ IU/ml in large dogs (>22 kg), $0.076{\pm}0.046$ IU/ml in small dogs (<22 kg), $0.085{\pm}0.036$ IU/ml in neutered dogs and $0.056{\pm}0.022$ IU/ml in intact dogs. The concentration of TRAP in the neutered dogs was significantly (p<0.05) higher than the intact dogs. This data can be used baseline data for a comparison with joint with injury of cranial cruciate ligament.

본 연구에서는 십자인대 손상견의 무릎관절액의 TRAP의 농도와 비교 할 수 있는 정상 관절액의 TRAP 농도의 지표를 제시 하기 위하여, 정상 무릎 관절액의 TRAP의 농도를 측정하였다. 십자인대 손상이 없는 23두 건강한 잡종견(14두 노령견과 9두 성견)을 실험견으로 선택하고 성별, 나이, 몸무게로 구별하였다. 무릎 관절액을 채취 한뒤, Lang method로 관절액의 TRAP을 측정하였다. 측정된 TRAP의 농도를 성별, 나이, 몸무게 등으로 구별하여 $Mean{\pm}SD$로 표시 하였으며, 유의성 차이를 T-test로 구별하였다. 노령견(14두, 2년이상) TRAP의 농도는 $0.083{\pm}0.039\;IU/ml(mean{\pm}SD$, 성견(9두, 2년 이하) $0.064{\pm}0.023\;IU/ml$, 대형견(15두, >22 kg) $0.075{\pm}0.028\;IU/ml$, 소형견(8두, <22 kg) $0.076{\pm}0.046\;IU/ml$, 중성화견(15두) $0.085{\pm}0.036\;IU/ml$, 중성화 되지 않은견(8두) $0.056{\pm}0.022\;IU/ml\;(Mean{\pm}SD)$로 나타났으며, 중성화 견과 중성화 되지 않은 견에서만 유의성 있는 차이 (P<0.05)가 보였다. 본 연구의 정상견에서 TRAP 농도는 십자인대의 손상견과 비교할수 있는 지표로 활용할 수 있을 것으로 사료된다.

Keywords

References

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