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The Pharmaceutical Society of Korea (대한약학회)
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The Cytotoxicity of Eutigosides from Eurya emarginata Against HL-60 Promyelocytic Leukemia Cells

  • Park Soo Yeong (Department of Medicine, Cheju National University, Technology Innovation Center, Cheju National University) ;
  • Yang Hong Chul (Department of Chemistry, Cheju National University) ;
  • Moon Ji Young (Department of Chemistry, Cheju National University) ;
  • Lee Nam Ho (Department of Chemistry, Cheju National University) ;
  • Kim Se Jae (Technology Innovation Center, Cheju National University) ;
  • Kang Ji Hoon (Department of Medicine, Cheju National University) ;
  • Lee Young Ki (Department of Medicine, Cheju National University) ;
  • Park Deok Bae (Department of Medicine, Cheju National University) ;
  • Yoo Eun Sook (Department of Medicine, Cheju National University) ;
  • Kang Hee Kyoung (Department of Medicine, Cheju National University)
  • Published : 2005.09.01
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Abstract

Two phenolic glucosides, eutigoside Band eutigoside C were isolated from the fresh leaves of Eurya emarginata. These two phenolic glucosides exerted a significant inhibitory effect on the growth of HL-60 promyelocytic leukemia cells. Furthermore, when the HL-60 cells were treated with eutigoside C, several apoptotic characteristics such as DNA fragmentation, morphologic changes, and increase of the population of sub-G1 hypodiploid cells were observed. In order to understand the mechanism of apoptosis induction by eutigoside C, we examined the changes of Bcl-2 and Bax expression levels. The eutigoside C reduced BcI-2 protein and mRNA levels, but slightly increased Bax protein and mRNA levels in a time-dependent manner. When we examined the activation of caspase-3, an effector of apoptosis, the eutigoside C increased the expression of active form (19-kDa) of caspase-3 and the increase of their activities was demonstrated by the cleavage of poly (ADP-ribose) polymerase, a substrate of caspase-3, to 85-kDa. The results suggest that the inhibitory effect of eutigoside C from E. emarginata on the growth of HL-60 appears to arise from the induction of apoptosis via the down-regulation of BcI-2 and the activation of caspase.

Keywords

References

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