Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics of Staphylococcus aureus Isolated from Patients with Diarrhea.

설사 환자에서 분리된 Staphylococcus aureus의 특성

  • Park Eun Hee (Busan Metropolitan City Institute of Health & Environment) ;
  • Min Sang Gi (Busan Metropolitan City Institute of Health & Environment) ;
  • Lee Ju Hyeoun (Busan Metropolitan City Institute of Health & Environment) ;
  • Park Yon Koung (Busan Metropolitan City Institute of Health & Environment) ;
  • Jeong Gu Young (Busan Metropolitan City Institute of Health & Environment) ;
  • Bin Jae Hun (Busan Metropolitan City Institute of Health & Environment)
  • 박은희 (부산광역시 보건환경연구원) ;
  • 민상기 (부산광역시 보건환경연구원) ;
  • 이주현 (부산광역시 보건환경연구원) ;
  • 박연경 (부산광역시 보건환경연구원) ;
  • 정구영 (부산광역시 보건환경연구원) ;
  • 빈재훈 (부산광역시 보건환경연구원)
  • Published : 2005.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The major causative bacteria of food poisoning were Salmonella spp. $(35.6\%)$, Staphylococcus aureus $(11.3\%)$ and Vibrio parahaemolyticus $(3.2\%)$ in our country. In this study we attempted isolation of S. aureus from stools of patients with diarrhea. Sixty-four strains $(9.1\%)$ were isolated from 704 the stools of patients with diarrhea. The enterotoxin was detected from 29 isolates $(45.3\%)$: 24 isolates $(37.5\%)$, 3 isolates $(4.7\%)$ and 2 isolates $(3.1\%)$ were A, B and C type, respectively. In the antibiotic susceptibility, 63 isolates $(98.4\%)$ were resistant to penicillin, 60 isolates $(93.8\%)$ to ampicillin, 35 isolates $(54.7\%)$ to erythromycin, 32 isolates $(50.0\%)$ to gentamycin, 22 isolates $(34.4\%)$ to tetracycline and 20 isolates $(31.3\%)$ to oxacillin. All of S. aureus isolates were susceptible to chloramphenicol and vancomycin, 20 isolates $(31.3\%)$ were methicillin-resistance S. aureus (MRSA). MRSA isolation rate was higher in male $(35.7\%)$ than female $(26.3\%)$. With the exception of two isolates which were resistant only to penicillin, sixty-one isolates were multiple antibiotic resistance.

부산광역시 관내 병원에 내원한 설사환자 704명의 분변으로부터 64건의 황색포도상구균을 분리하였다. 황색포도상구균의 평균 분리율은 $9.1\%$였으며, 5월에 가장 높은 16건$(16.8\%)$이 분리되었으나 계절에 상관없이 꾸준히 분리되었다. 분리된 황색포도상구균 64균주 중 $45.3\%$인 29균주의 황색포도상구균이 장독소를 생산하였으며, 장독소 유형별로 A형이 24균주$(37.5\%)$, B형이 3균주$(4.7\%)$, C형이 2균주$(3.1\%)$순이었다. 또한 15종의 항생제에 대한 내성율은 penicillin과 ampicillin에 대하여 각각 $95.4\%$와 $93.8\%$로 매우 높은 내성율을 나타내었으나, chloramphenicol과 vancomycin에 대하여는 $100\%$ 감수성이었다. Oxacillin에 내성을 나타내는 MRSA은 $31.3\%$였으며, vancomycin에 내성을 나타내는 VRSA은 $0\%$였다. 성별과 연령에 따른 MRSA의 분리율은 남자가 $35.7\%$ (15균주/42균주), 여자는 $26.3\%$(5균주/19균주)로 남자가 더 높았으며, 연령별로는 9세 이하 5명(남자 4명, 여자 1명), 20대 1명(남자 1명), 30대 2명(남자 2명), 50대 4명(남자 3명, 여자 1명), 60세 이상 8명(남자 5명, 여자 3명)이었다. 10대와 40대에서는 MRSA가 분리되지 않았다. 공시 항생제에 대한 내성양상은 분리된 64주 중 61주의 균이 최소 2종류 이상의 약제에 대하여, 최고 12종류의 약제에 대하여 내성을 나타내는 다양한 다제내성형을 나타내었다.

Keywords

References

  1. Atanassova, V., A. Meindl and C. Ring. 2001. Prevalence of Staphylococcus aureus and Staphylococcal enterotoxins in raw pork and uncooked smoked ham-a comparison of classical culturing detection and RFLP-PCR. Int. J. Food Microbiol. 68, 105-113 https://doi.org/10.1016/S0168-1605(01)00479-2
  2. Brown, M. H. 1982. Meat microbiology. pp. 269-486, Applied Science Publishers. London and New York
  3. Kim B. S., Y. S. Lee, J. I. Yoo, J. K. Lee, J. O. Cha and E. S. Shin. 2001. Analysis of mec regulation gene and PFGE on Staphylococcus aureus isolates from tertiary hospitals in Korea. The Report of NIH 38, 43-51
  4. Casman, E. P., R. W. Bennett, A. E. Dorsey and J. A. Issa. 1967. Identification of a fourth Staphylococcal enterotoxin, enterotoxin D. J. Bacteriol. 94, 1875-1882
  5. Kim C. M., S. H. Park, D. H. Lee, H. S. Kwak, Y. S. Kang, Y. C. Park, S. K. Yoon, S. H. Jeong and J. Y. Moon. 2000. Studies on the Risk Assessment and Management of Food-borne Microorganisms. The annual Report of KFDA 4, 655-656
  6. Kim D. H., K. R. Kwon, K. H. Lee, Y, R. Ju, K. S. Oh and H. S. Kark. 1988. Study on Staphylococcal enterotoxin. The Report of NIH 25, 297-307
  7. Park H. O., C. M. Kim, G. J. Woo, S. H. Park, D. H. Lee, E. J. Chang and K. H. Park. 2001. Mornitoring and Trends Analysis of Food Poisoning Outbreaks Occurred in Recent Years in Korea. J. Fd Hyg. Safety 16, 280-294
  8. Hepner, E. P. 1980. Food poisoning and Salmonella infections in England and Wales, 1976-1978. Publ. Hlth. Lond. 94, 337-349
  9. Jeong H. Y., S. D. Lee, S. J. Jang, S. H. Park, J. Y. Chang, C. S. Min, H. S. Shin, K H. lee, K W. Kim and D. K Rhee. 2001. Monitoring on the Bacterial Resistance to Antibiotics (ll). The annual Report of KFDA 5, 321-329
  10. Jeong H. Y., S. J. Jang, S. D. Lee, S. H. Park, C. S. Min, S. Y. Lee, K H. Lee, J. E. Lee, M. S. Lee and K. W. Lee. 2002. Monitoring on the Bacterial Resistance to Antibiotics. The annual Report of KFDA 6, 222-229
  11. Jevons M. P. 1961, 'Cellbenin' -resistant staphylococci. BMJ 1, 124-126
  12. Kim J. I., S. K. Park, Y. H. Min, S. H. Bing, Y. S. Heo and B. E. Lee. 2004. Investigate the Isolation and Identification of Pathogenic Bacteria from diarrheal patients, The Report of Daejon Metropolitan City Health and Environment Reserch Institute 4, 7-20
  13. Lowy F. D. 1998. Staphylococcus aureus infections. N. Engl. J. Med. 339, 520-532 https://doi.org/10.1056/NEJM199808203390806
  14. Martin M. D., P. M. Orwin and P. M. Schievert. 2000. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 13, 16-34 https://doi.org/10.1128/CMR.13.1.16-34.2000
  15. National Committee for Cinical Laboratory Standards. 1993. Performance Standards for Antimicrobial Disk Susceptibility Tests (Approved Standard, M2-M5). Villanova, PA, National Committee for Clinical Laboratory Standards
  16. Park S. G., Y. O. Hwang, J. H. Jung and K. M. Lee. 2001. Biological Characteristics of Staphylococcus aureus Isolated from Food-Borne Patients in Seoul. J. Fd Hyg. Safety 16, 159-167
  17. Takeshige K, K. Watanabe, H. Igarashi, M. Shingak and T. Terayama. 1983. Detection of Staphylococcus au reus in bovine mastitis and some characteristics with special reference to enterotoxin producibility and coagulase types of isolates. Jpn. J. Vet. Sci. 45, 355-362 https://doi.org/10.1292/jvms1939.45.355
  18. Tanaka Y., A. Adachi, A. Ashimoto, H. Kishimoto, R. Teshima and K. Yamamoto. 1992. Drug-resistant Staphylococcus aureus contamination in the ward environment. Kanasenshogaku Zasshi 66, 1270-127
  19. Kang Y. S., S. Y. Yoon, S. H. Jwa, D. H. Lee, G. J. Woo, Y. S. Park and C. M. Kim. 2002. Prevalence of Staphylococcus aureus in Kimbop. J. Fd Hyg. Safety 17, 31-35
  20. Lee Y. S., H. B. Kim, J. I. Yoo, S. J. Yang, C. M. Sa, Y. H. Choi and B. S. Kim. 1999. Molecular epidemiology of methicillin resistant Staphylococcus aureus in Korea. The Report of NIH 36, 67-76
  21. Lee Y. S., J. O. Cha, Y. H. Jung, Y. H. Choi, Y. S. Yang, H. B. Kim, J. I. Yoo and B. S. Kim. 2000. Molecular epidemiological study on Staphylococcus aureus isolates from blood specimen in Korea. The Repory of National Institute of Health 37, 53-60
  22. Frazier W. C. and D. C. Westhoff. 1978. Food microbiology. pp. 432-438, 3rd ed., MacGraw-Hill Book Company