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천연보존제로서 한국산 무 및 종자 단백질의 생화학적 특성 : 항유전독성 및 세포독성에 미치는 영향

The Biochemical Properties of Korean radish (Raphanus sativus L.) and Its Seed Protein as a Natural Preservative : The Influences on Antigenotoxicity and Cytotoxicity

  • Choi Yoon-Hyeok (Institute of Bioscience and Technology, Handong Global University) ;
  • Hwang Cher-Won (School of Liberal arts, Handong Global University)
  • 발행 : 2005.08.01

초록

본 연구는 한국산 무 및 그 종자단백질을 추출 및 분리하여 3T3 세포주에 대해 항유전독성과 세포독성에 미치는 영향과 항균활성을 검토하였다. 3T3 세포주에 직접발암원인 $100{\mu}g/ml$ MNNG를 $10{\mu}l$씩 투여한 후 10mg/ml의 무추출물(RJ)과 1mg/ml의 종자단백질(RSP)을 $10{\mu}l$씩 투여하였을 때, 각각 $43\%$$30\%$의 항유전독성을 보였다. 또한 lactose dehydrogenase (LDH) 정량 분석을 이용하여, 3T3 세포주에 대한 다양한 농도의 RJ 및 RSP를 투여한 후 세포독성을 관찰 한 결과 세포독성은 관찰되지 않았다. 한편 이미 오염된 화장품에 대해 10 mg/ml 의 RJ와 1mg/ml의 RSP를 투여하였을 때, 세균성오염화장품 시료(C1)에 대해서 RSP가 $85\%$의 항균활성을 보였으며 진균성오염화장품 시료(C4)에 대해서 RSP는 $79\%$, RJ는 $76\%$의 항균활성을 보였다.

In this study, we report antigenotoxicity and cytotoxicity of Korean Radish extract (RJ) and its seed protein (RSP) to non-tumoral 3T3 cell line. In the case of antigenotoxicity, each cell line was treated with $10{\mu}l\;of\;100{\mu}g/ml$ N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) before adding $10{\mu}l$of 10mg/ml RJ and 1mg/ml RSP to the cell. Both RJ and RS were shown $30\%\;and\;43\%$ of antigenotoxicity respectively. As a result of quantitative analysis for lactose dehydrogenase (LDH), no cytotoxic activity against 3T3 cells was detected when the cells were treated with various concentrations of RJ and RSP, RSP showed $85\%$ of antimicrobial activity against cosmetic sample (C1) assumed as contaminated by bacteria. RSP and RJ showed $79\%\;and\;76\%$ of antimicrobial activities repectively on another cosmetic sample (C4, contaminated by fungi) were treated with 10mg/ml RJ and 1 mg/ml RSP

키워드

참고문헌

  1. An. B. J. 1999. The meterial of natural anti-bacterial agents for the food preservative. Food Industry and Nutrition, 4, 5-16
  2. Choi, J. W., J. H. Park, S. T. Ji. O. B. Choi, H. K. Shin. 1999. Antigenotoxic effect of dominant bacteria isolates from Kimchi in vitro. Korean J. Food Sci. Technol., 31, 1071-1076
  3. Gisell, R. F., C. B. Lourdes, L. N. Marcelam, P. Ana, M. Mudry, G. E. Prieto, A. C. Marta. 2002. DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives. Toxicol. Lett., 132, 109-115 https://doi.org/10.1016/S0378-4274(02)00039-5
  4. Hwang, C. W. 2003. Antifungal activity of Korean Radish (Raphanus sativus L.) extract against pathogenic plant. J. of Life Science, 13, 223-229 https://doi.org/10.5352/JLS.2003.13.2.223
  5. Jang, K. W., S. H. Park, S. D. Ha. 2003. Technology trends in functional foods. Food Sci. and Industry, 36, 8-16
  6. Jeong, M. S., G. S. Lee, H. J. Chae. 2004. In vitro biological activity assay of ethanol extract of radish. J. Korean Soc. Appl. BioI. Chem., 47, 67-71
  7. Jung, D. H. 1998. In biological efficacy of food, Seonjin Munwhasa, Seoul, 72-74
  8. Kim, Y. R., S. H. Ahn, B. D. Choi, S. J. Kang, G. W. Shin, M. J. Oh, T. S. Jung. 2004. In vitro examination of chondroitin sulfates extracted Midduck (Styela clava) and Munggae Tunics (Halocynthia roretzi) as a cosmetic material. J. Korean Soc. Food Sci. Nutr., 33, 646-652 https://doi.org/10.3746/jkfn.2004.33.4.646
  9. Park, J. H., H. K. Shin, C. W. Hwang. 2001. New antimicrobial activity from Koreans Radish seeds (Raphanus sativus L.). J. Microbiol. Biothechnol., 11, 337-341
  10. Sabalitschka, T. 1930. Application of ethyl p-hydroxybenzoate in maintenance of sterility, in sterilization and in disinfection. Archives of Pharmacy, 268, 653 - 673 https://doi.org/10.1002/ardp.19302680905
  11. Schafer Jr, E. W., W.A. Bowles, Jr. 1985. Acute oral toxicity and repellency of 933 chemicals to house and deer mice. Arch. Envron. Contam. Toxicol., 14, 111 - 129 https://doi.org/10.1007/BF01055769
  12. Sohn, E. S., S. W. Kim, J. S. Kang, S. P. Lee. 2004. Technology trend and patent information analysis of cosmetic materials derived from natural products. Applied Chemistry, 8, 466-469
  13. Souria, E., Gh. Amin, H. Farsam, S. Andaji. 2003, The antioxidant activity of some commonly used vegetables in Iranian diet. Fitoterapia, 75, 585-588 https://doi.org/10.1016/j.fitote.2004.04.007

피인용 문헌

  1. Study of Distance Relationships among Domestic Radish (Raphanus sativus L.) by Analyzing its Anti-fungal Protein Gene. vol.17, pp.9, 2007, https://doi.org/10.5352/JLS.2007.17.9.1294