Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Kinds and Concentrations of Cryoprotectants, Trehalose, Sucrose-Addition in Cryoprotectants on the Survival Rates of Vitrification-Thawed Porcine IVM/IVF Embryos

돼지 난포란의 내동제의 종류와 농도 및 Trehalose, Sucrose등의 첨가가 Vitrification 동결 융해 후 생존율 및 수정율에 미치는 영향

  • Lim J. G. (College of Agriculture and Life Science, Chungnam National University) ;
  • Quan J. H. (College of Veterinary Medicine, Chungnam National University) ;
  • Lee K. S. (College of Agriculture and Life Science, Chungnam National University) ;
  • Kim S. K. (College of Veterinary Medicine, Chungnam National University)
  • Published : 2005.08.01
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Abstract

This study examines the effects of kinds and concentrations of cryoprotectants on the survival rate of vitrification-thawed porcine oocytes, together with the effects on survival, in vitro fertilization and development of immature oocytes. 1. The developmental rate of oocytes to MII and diploid stage when the vitrification-thawed of recovered immature oocytes cultured for 0, 15, 30 and 40h were cultured for 0, 15, 30 and 40h were $56.7\%,\;53.3\%,\;63.3\%,\;65.0\%\;and\;23.3\%,\;18.3\%,\;10.0\%,\; 3.3\%$, respectively. The in vitro development to MII stage were lower than the control group $(78.2\%)$, but higher fo. diploid stage $(5.5\%)$. 2. When the vitrification of immature oocytes after being culture for 0, 15, 30 and 40 hours, the survival rate were $34.0\%,\;26.0\%,\;18.0\%\;and\;10.0\%$ respectively. This result was lower than that of the control group $(60.0\%)$. 3. When the fertilization of the vitrified immature oocytes after being culture for 0, 15, 30 and 40 hours, the in vitro fertilization rate were $60.0\%,\;54.0\%,\;48.0\%,\;38.0\%$, and developmental rates were $26.0\%,\;18.0\%,\;8.0\%,\;4.0\%$, respectively. This results were lower than the control group $(78.0\%\;and\;38.0\%)$. 4. When the fertilization of the immature oocytes after being culture for $0\~15$ hours vitrified with EDS and ETS, the fertilization and developmental rates were $50.0\%,\; 22.0\%$ and $46.0\%,\;18.0\%$, respectively. This results were lower than the control group $(74.0\%\;and\;38.0\%)$.

본 연구는 돼지 난포란의 vitrification 동결 시 내동제의 종류 및 농도가 생존율에 미치는 영향과 수정 후 체외발생율을 조사하고자 수행하였다. 1.0, 15, 30 및 40시간 성숙 배양시킨 난포란을 vitirfication 동결보존 후의 MII로의 발생율은 각각 $56.7\%,\;53.3\%,\; 63.3\%,\;65.0\%$였으며, diploid로의 발생율은 $23.3\%,\;18.3\%,\;10.0\%,\;3.3\%$로서 대조군의 ME 단계의 $78.2\%$에 비해 낮게 나타났으며 diploid 단계의 $5.5\%$에 비해서는 높은 체외성숙율을 나타냈다. 체외발생율은 초기의 미숙 난포란일수록 높은 체외성숙율을 나타냈다. 2. 미성숙 난포란을 회수 후 0, 15, 30 및 40시간 성숙 배양시킨 난포란을 vitirfication 동결 후 융해하였을 때 생존율은 각각 $34.0\%,\;26.0\%,\;18.0\%,\; 12.0\%,\;10.0\%$로서 비동결 난포란의 $60.0\%$에 비해 낮게 나타났지만 비교적 양호한 생존율을 나타냈다. 3. 0, 15, 30 및 40시간 성숙 배양시킨 미성숙 난포란을 vitrification 동결 융해 후 수정시켰을 때 체외수정율은 $60.0\%,\;54.0\%,\; 48.0\%,\;38.0\%$였으며, 배반포로의 체외발생율은 각각 $26.0\%,\;18.0\%,\; 8.0\%,\;4.0\%$로서 비동결 대조군의 $78.0\%$와 $38.0\%$에 비해 낮은 체외수정율과 체외발생율을 나타냈다. 4. 돼지 난포란을 EDS와 ETS 액으로 vitrifiestion 동결운해 후 $0\~15$ 시간 배양한 다음 체외수정시켰을 때 체외 수정율과 발생율은 각각 $50.0\%,\;22.0\%$ 및 $46.0\%,\;18.0\%$로서 대조군의 $74.0\%$와 $38.0\%$에 비해 낮게 나타났다.

Keywords

References

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