Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle

한우 및 젖소에서 체외 수정란 생산과 신선 및 동결 수정란 이식 결과

  • Kim Y. J. (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim H. C. (College of Veterinary Medicine, Chonbuk National University) ;
  • Seo S. H. (Jeonju-Zoo) ;
  • Jeong K. N. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do) ;
  • Kim Y. S. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do) ;
  • Lee H. R. (College of Veterinary Medicine, Chonbuk National University) ;
  • Shin D. S. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do) ;
  • Jo S. W. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do) ;
  • Kim S. H. (College of Veterinary Medicine, Chonbuk National University)
  • 김용준 (전북대학교 수의과대학) ;
  • 김희천 (전북대학교 수의과대학) ;
  • 서세현 (전주동물원) ;
  • 정구남 (전라북도 축산진흥연구소 종축시험소) ;
  • 김용수 (전라북도 축산진흥연구소 종축시험소) ;
  • 이해리 (전북대학교 수의과대학) ;
  • 신동수 (전라북도 축산진흥연구소 종축시험소) ;
  • 조성우 (전라북도 축산진흥연구소 종축시험소) ;
  • 김수희 (전북대학교 수의과대학)
  • Published : 2005.08.01
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Abstract

The ovaries from Hanwoo and Holstein were collected from labattoir and transferred to laboratory. Oocytes were aspirated and incubated in $CO_2$ incubator for 24 hours for maturation. Oocytes were coincubated with the sperms for 5 hours. Cleaved oocytes were selected 48 hours after coincubation and half of the medium was changed newly every 48 hour until blastocyst formation. Cleavage rate and blastocyst rate were investigated according to different breeds and different status of cumulus cells surrounding the oocytes. Blastocysts were either transferred to the recipients or frozen until use. The result of embryo transfer with fresh or frozen embryos was investigated. The rate of male offspring following embryo transfer was also investigated. The rate of cleavage was $66.4\%$ for Hanwoo and $62.4\%$ for Holstein oocytes. The rate of cleavage according to status of oocyte was shown highest in the oocytes completely surrounded with cumulus cells and lowest in denuded oocytes for both Hanwoo and Holstein oocytes. The rate of blastocyst from cleaved oocytes was $40.6\%$ for Hanwoo and $36.9\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with fresh IVF embryos was $57.2\%$ for Hanwoo and $53.3\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with frozen IVF embryos was $40.9\%$ for Hanwoo and $36.4\%$ for Holstein. The rate of male calf produced by embryo transfer was $63.6\%$ for Hanwoo and $50.0\%$ for Holstein.

한우 및 젖소에서 체외 수정란을 생산하여 수란 우에 이식하기 위해 도축장에서 난소를 채취하여 난자를 회수한 후 체외 성숙, 체외 수정, 체외 배양과정을 거쳐 수정란을 생산하였다. 체외 수정시 난자는 난구세포 부착 정도에 따라 난할율을 조사하였고, 한우와 젖소에서 체외수정 후 배반포율을 조사하였다. 신선 및 동결 수정란을 수란우에 각각 이식하여 산자 분만에 따른 수태율을 조사하였다. 1. 한우 난소 2,021개에서 20,387개의 난자를 회수하여 체외 수정 후 난할된 난자수는 13,541개로서 $66.4\%$의 난할율을 나타내었고, 젖소는 난소 144개에서 1,784개의 난자를 회수하여 체외 수정 후 1,113개의 난자가 난할되어 $62.4\%$의 난할율을 나타내었다. 2. 한우와 젖소에서 난구세포가 온전히 둘러싸인 난자의 율은 각각 45.0, $62.0\%$이었고 난할율은 각각 81.0, $72.0\%$이었다. 난구세포가 일부 분리된 난자의 난할율은 한우, 젖소 각각 63.5, $51.3\%$이었다. 난구세포가 완전 분리된 난자의 난할율은 한우, 젖소 각각 41.0, $41.7\%$이었다. 3. 한우와 젖소 체외수정에서 난자가 배반포로 발달된 율은 전체 난자수에 비교하여 각각 27.0, $23.0\%$이었고, 난할된 난자수에 비교하여 각각 40.6, $36.9\%$이었다. 4. 신선 수정란을 이식한 결과, 한우에서는 278두에 이식하여 159두가 산자를 분만하여 $57.2\%$, 젖소에서는 15두에 이식하여 8두가 분만함으로써 $53.3\%$의 임신율을 나타내었다. 5. 동결 수정란을 이식한 결과, 한우에서는 44두에 이식하여 18두가 산자를 분만하여 $40.9\%$, 젖소에서는 11두에 이식하여 4두가 분만함으로써 $36.4\%$의 임신율을 나타내었다.

Keywords

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