Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Influence of Bakers' Yeast Cells on Protein Adsorption in Anion Exchange Expanded Bed Chromatography

  • Mei Chow Yen (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ti Tey Beng (Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ibrahim Mohammad Nordin (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ariff Arbakariya (Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia) ;
  • Chuan Ling Tau (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
  • Published : 2005.06.01
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Abstract

The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE $(\rho=1.2g/cm^3)$ from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to $15\%(w/v)$. Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

Keywords

References

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