Biotechnology and Bioprocess Engineering:BBE

  • 제10권3호
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  • Pages.262-269
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  • 2005
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

  • Ahn Jae-Il (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Jang In-Keun (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Opthalmology, Chung-Ang University Hospital) ;
  • Lee Doo-Hoon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Seo Young-Kwon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Yoon Hee-Hoon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Shin Youn-Ho (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Kim Jae-Chan (Department of Opthalmology, Chung-Ang University Hospital) ;
  • Song Kye-Yong (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Lee Hee-Gu (Molecular and Cell Biology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yang Eun-Kyung (R&D Center, Bioland Ltd.,) ;
  • Kim Ki-Ho (R&D Center, Bioland Ltd.,) ;
  • Park Jung-Keung (Department of Chemical and Biochemical Engineering, Dongguk University)
  • 발행 : 2005.06.01
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초록

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

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