Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Helicobacter pylori Strain 51 (Korean Isolate): Ordered Overlapping BAC Library, Combined Physical and Genetic Map, and Comparative Analysis with H. pylori Strain 26695 and Strain J99

  • KANG HYUNG-LYUN (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • LEE WOO-KON (Department of Microbiology, Gyeongsang National University College of Medicine, Research Institute of Life Science and Central Laboratory, Gyeongsang National University) ;
  • SONG JAE-YOUNG (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • CHOI SANG-HAENG (Korea Research Institute for Bioscience and Biotechnology) ;
  • PARK SEONG-GYU (Department of Microbiology, Gyeongsang National University College of Medicine) ;
  • RYU BOK-DEOK (Department of Microbiology, Gyeongsang National University College of Medicine) ;
  • LEE EUN-JOO (Department of Microbiology, Gyeongsang National University College of Medicine) ;
  • KIM JI-SUN (Korea Research Institute for Bioscience and Biotechnology) ;
  • PARK JEONG-UCK (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • BAIK SEUNG-CHUL (Department of Microbiology, Gyeongsang National University College of Medicine, Department of Gyeongsang Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • CHOI MYOUNG-BUM (Department of Pediatrics, and Gyeongsang National University College of Medicine) ;
  • YOUN HEE-SHANG (Department of Pediatrics, and Gyeongsang National University College of Medicine) ;
  • KO GYUNG-HYUCK (Department of Pathology, and Gyeongsang National University College of Medicine)
  • Published : 2005.08.01
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Abstract

We constructed a defined physical and genetic map of H. pylori strain 51, previously isolated from a Korean patient with a duodenal ulcer, by combining a restriction analysis by pulse-field gel electrophoresis with the construction of a BAC library. A Notl-digest of H. pylori strain 51 genome yielded seven fragments, from which the genomic size was estimated to be 1,698$\pm$24 kb. The BAC library was constructed from 50 to 200 kb fragments of HindIII-digested genomic DNA. From 700 BAC clones, an ordered overlapping maxi-set of 82 BAC clones was assembled that covered the entire genome. The positions of 15 genes were localized in the strain 51 genome with 4-22 kb of resolution and were compared with their orthologues in strain 26695 and strain J99. The arrangement of the 15 genes was identical in strain 51 and strain J99, except for flaA and hpaA. The plasticity zone of strain 51, like that of strain J99, was located in the single region, and was shorter than those of strain 26695 and strain J99. The strain 51 plasticity zone consisted of ORFs common only to strain 51 and J99 or to strain 51 and 26695, as well as strain 51-specific ORFs. Three genetic translocations and/or inversions were found between orthologue ORFs in strain 51 and strain J99. These results show that the chromosomal organization of strain 51 differs from Western strains such as strain 26695 and strain J99.

Keywords

References

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