Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Large-Scale Fermentation for the Production of Teicoplanin From a Mutant of Actinoplanes teichomyceticus

  • LEE JAE-CHAN (Korea Research Institute of Bioscience & Biotechnology) ;
  • MIN JUNG-WON (Korea Research Institute of Bioscience & Biotechnology) ;
  • PARK DONG-JIN (Korea Research Institute of Bioscience & Biotechnology) ;
  • SON KWANG HEE (Korea Research Institute of Bioscience & Biotechnology) ;
  • YOON KI-HONG (School of Food Science & Biotechnology, Woosong University) ;
  • PARK HAE-RYONG (Division of Food Science and Biotechnology, Kyungnam University) ;
  • PARK YOUNG-SOO (Central Research Center, Kunwha Pharmaceutical Co.) ;
  • KWON MU-GIL (Central Research Center, Kunwha Pharmaceutical Co.) ;
  • LEE JUNG-MIN (Korea Research Institute of Bioscience & Biotechnology) ;
  • KIM CHANG-JIN (Korea Research Institute of Bioscience & Biotechnology)
  • Published : 2005.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Mutation and its pilot-scale fermentation were conducted for the production of teicoplanin from Actinoplanes teichomyceticus. The fermentation medium was optimized by replacement and Plackett-Burman experimental design. A maximum production of 1,500 mg/l teicoplanin was obtained by pilot-scale fermentation in an optimized medium containing (g/l): 30 g maltodextrin, 5 g glucose, 5 g yeast extract, 5 g soybean meal, 0.5 g $MgSO_4{\cdot}7H_2O$, 0.1 g NaCl, 0.1 g $CaCl_2{\cdot}2H_2O$, and 50 g Diaion HP-20. The production of teicoplanin was improved 3-fold from the parental strain by mutation, media optimization, and fermentation, and laboratory-scale fermentation was successfully demonstrated in a pilot-scale fermenter for the industrial production of teicoplanin.

Keywords

References

  1. Ashwell, G. 1957. Colorimetric analysis of sugars, pp. 84-85. In S. P. Collowick and N. O. Kaplan (eds.), Methods in Enzymology, vol. 3. Academic Press, New York, U.S.A
  2. Choi, D. and K. Cho. 2004. Effect of carbon source consumption rate on lincomycin production from Streptomyces lincolnensis. J. Microbiol. Biotechnol. 14: 532-539
  3. Heydom, A., S. J. Trine, and J. Nielsen. 1999. Growth and production kinetics of a teicoplanin producing strain of Actinoplanes teichomyceticus. J. Antibiot. 52: 40-44 https://doi.org/10.7164/antibiotics.52.40
  4. Jin, Z. H., M. R. Wang, and P. L. Cen. 2002. Production of teicoplanin by valine analogue-resistant mutant strains of Actinoplanes teichomyceticus. Appl. Microbiol. Biotechnol. 58: 63-66 https://doi.org/10.1007/s00253-001-0872-9
  5. Kim, D.-M., D.-W. Kim, J.-H. Lee, K.-H. Park, L.-M. Day, and D. F. Day. 1997. Development of constitutive dextransucrase hyper-producing mutants of Leuconostoc mesenteroides using the synchrotron radiation in the 701,000 eV region. Biotechnol. Tech. 11: 319-321 https://doi.org/10.1023/A:1018471429067
  6. Kim, S.-J., G.-J. Kim, D.-H. Park, and Y.-W. Ryu. 2003. High-level production of astaxanthin by fed-batch culture of mutant strain Phaffia rhodozyma AJ-6-1. J. Microbial. Biotechnol. 13: 175-181
  7. Lee, H.-W., J. Yoon, J. H. Sohn, K.-H. Lee, B.-I. Yeh, D.-W. Park, H.-W. Kim, and J.-w. Choi. 2003. Detection of MecA gene in clinical isolates of Staphylococcus aureus by multiplex-PCR, and antimicrobial susceptibility of MRSA. J. Microbiol. Biotechnol. 13: 354-359
  8. Lee, J.-C., H.-R. Park, D.-J. Park, H.-B. Lee, Y.-B. Kim, and C.-J. Kim. 2003. Improved production of teicoplanin using adsorbent resin in fermentations. Lett. Appl. Microbial. 37: 196-200 https://doi.org/10.1046/j.1472-765X.2003.01374.x
  9. Lee, I.-C., H.-R. Park, D.-I. Park, K.-H. Son, K.-H. Yoon, Y.-B. Kim, and C.-I. Kim. 2003. Production of teicoplanin by a mutant of Actinoplanes teichomyceticus. Biotechnol. Lett. 25: 537-540 https://doi.org/10.1023/A:1022842203917
  10. Lee, I.-C., H.-R. Park, D.-I. Park, Y.-B. Kim, and C.-I. Kim. 2003. Separation and purification of teicoplanin by Diaion HP-20 and concanavalin A chromatography. Kor. J. Appl. Microbiol. Biotechnol. 31: 201-204
  11. Parenti, F., G. Beretta, M. Berti, and V. Arioli. 1978. Teicomycins, new antibiotics from Actinoplanes teichomyceticus novo sp. J. Description of the producer strain, fermentation studies and biological properties. J. Antibiot. 31: 276-283 https://doi.org/10.7164/antibiotics.31.276
  12. Plackett, R. L. and J. P. Burman. 1946. The design of optimum multifactorial experiments. Biometrica 33: 305-325 https://doi.org/10.1093/biomet/33.4.305
  13. Ryu, Y. G, W. Jin, J. Y. Kim, J. Y. Kim, S. H. Lee, and K. J. Lee. 2004. Stringent factor regulates antibiotics production and morphological differentiation of Streptomyces clavuligerus. J. Microbiol. Biotechnol. 14: 1170-1175
  14. Sosio, M., H. Kloosterman, A. Bianchi, P. Vreugd, L. Dijkhuizen, and S. Donadio. 2004. Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. Microbiology 150: 95 - 102 https://doi.org/10.1099/mic.0.26507-0